摘要
研究了在MRS培养基中添加不同营养物质对嗜热链球菌生长的影响,进一步采用正交试验优化筛选了嗜热链球菌的增殖培养基。结果表明:在MRS培养基中添加玉米浆、番茄汁、啤酒液、乳糖可显著促进试验菌株的细胞生长(P<0.01),而添加蛋白胨、酵母浸膏、CaCl2对细胞生长无显著影响(P>0.01);利用L934正交试验筛选出增殖培养基的最佳配比为:在MRS培养基中添加0.3%玉米浆、7.5%番茄汁、5.0%啤酒液、1.0%乳糖;试验菌株在增殖培养基中经37℃培养24h,活菌数可达4.69×109cfu/ml,较对照MRS培养基的活菌数提高40.78倍。
Effects of adding different nutriments in MRS medium on the growth of S.thermophilus ST-2 were examined.The optimum enrichment medium of S.thermophilus ST-2 was screened by orthogonality test.The results showed that adding maize hydrolyzates,tomato juice,beer,lactose in MRS medium obviously promoted the growth of S.thermophilus ST-2(P<0.01),but adding pepton,yeast extracts,CaCl2 in MRS medium exerted insignificantly effects on the growth of S.thermophilus ST-2(P>0.01).The optimum enrichment mediums obtained by orthogonality test consisted of adding maize hydrolyzates0.3%,tomato juice7.5%,beer5.0%,lactose1.0% in MRS medium.The living number of S.thermophilus ST-2 cultivated in the optimum enrichment medium for 24 hours at 37℃ was 4.69×109cfu/ml.Compared to controlling MRS medium,40.78 times of increase in the viable counts of bacteria was observed.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2002年第5期60-62,共3页
Food Science