摘要
应用脂质过氧化法和荧光探针法研究了蜂胶对氧自由基致鼠红细胞脱膜氧化损伤的保护作用。结果表明,蜂胶能显著地抑制氧自由基引发的鼠红细胞膜脂质过氧化反应,脂质过氧化产物丙二醛(MDA)含量显著下降,呈现量效关系。用Fenton反应产生的羟基自由基攻击红细胞膜后,膜脂的流动性显著下降,当红细胞膜经40μg/ml的蜂胶预先处理后,膜脂的流动性显著提高,表明蜂胶对氧自由基引发的鼠红细胞氧化损伤有显著的保护作用。
Both lipid peroxidation and fluorescence polarization were used to dertimne the protective action of propolis extract on the effect of rat erythrocyte ghost injury induced by oxygen-centred free radicals.The results showed that propolis could significantly inhibit lipid oxidation of rat erythrocyte ghost induced by action oxygen free radicals(peroxy radical and hydroxy radical)with dose-dependent effect.The erythrocyte ghost oxidative damage was induced by hydroxy free radical which could decrease membrance fluidity.But propolis(40μg/ml)could improve the membrance fluidity and inhibit the lipid peroxidation.These results showed that propolis could prevent lipid peroxidation in rat erythrocyte ghost damage induced by oxygen free dadicals.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2002年第5期118-120,共3页
Food Science
基金
陕西省自然科学基金资助项目(2000SM16)
