摘要
通过接种 1日龄AA肉用型鸡和 11日龄SPF鸡胚 (蛋用型鸡 )对 4个J亚群禽白血病病毒 (ALV J)中国分离株的致病性进行了研究。在连续观察的 6个月中 ,只有从病鸡分离到的SD990 2株ALV J可诱发急性髓细胞瘤(ML)。在SD990 2株病毒人工接种的 12只 1日龄的AA肉用型鸡中 ,有 9只分别在 2 2～ 38日龄死亡 ,其肝、脾显著增生性肿大 ,并显现弥散性分布的细小的灰白色结节 ,心肌上可见许多大小不一的肿瘤结节。病理组织切片中 ,在肝脏、心肌和骨骼肌等组织中均可见胞浆中大量充满嗜酸性颗粒的髓细胞样肿瘤细胞。其余 3株病毒感染的肉用型鸡无肉眼可见的肿瘤性变化 ,生长和临床表现正常。显然 ,SD990 2株ALV J为急性转化型病毒 ,而SD990 1、YZ990 1和YZ990 2这 3株病毒仍属致病性较弱的非转化型病毒。 11日龄的SPF鸡胚 (蛋用型鸡 )在接种这 4株病毒后孵出的鸡 ,在近 7个月的连续观察中 ,没有发现肿瘤性变化 ,其生长和临床表现基本正常 ,表明这 4株中国株ALV
The pathogenicity of 4 Chinese isolated strains of subgroup J Avian leukosis viruses (ALV J), SD9901, SD9902, YZ9901 and YZ9902, was studied. The results showed that in these 4 Chinese strains, only SD9902 strain induced death with Myloid Leukosis(ML) and among 12 AA meat type chickens inoculated with SD9902, 9 chickens died between 22 days and 38 days respectively, after inoculation at 1 day old. Chickens inoculated with the other 3 strains during the period of 6 months did not died. These results suggested SD9902 stain of ALV J was an acutely transforming virus, but SD9901, YZ9901 and YZ9902 stains were non transforming viruses. All 4 Chinese isolated strains did not induced tumors in SPF chickens (egg type) during 7 months even viruses were injected into 11 day old embryos.
出处
《中国农业科学》
CAS
CSCD
北大核心
2002年第4期430-433,T002,共5页
Scientia Agricultura Sinica
基金
国家自然科学基金资助项目 (3 0 0 40 0 11)
