摘要
目的 构建人KIR2DL4-IgFc段融合蛋白基因真核细胞表达载体。方法 用RT-PCR从孕妇蜕膜组织单个核细胞的总mRNA中逆转录扩增KIR2DL4胞外段cDNA,经Nhe I和Bam HI双酶切后,定向插入真核细胞表达载体CD51negl中,然后经酶切和测序鉴定。结果 限制性内切酶酶切和序列分析表明已成功构建CD51negl-KIR2DL4载体。结论 本研究成功构建KIR2DL4-IgFc融合蛋白真核细胞表达载体,为研究KIR2DL4与其配体之间的关系奠定了基础。
ve To construct eukaryotic expression vector of human KIR2DL4-IgFc fusion protein gene. Methods Total cell RNA was extracted from maternal decidual mononuclear cells and the cDNA was amplified by RT-PCR; the cDNA fragments were inserted into the eukaryotic expression vector CD51negl and the recombined plasmid was identified by restriction endonucleases digestion and sequencing. Re-sults After restriction endonucleases treatment and sequencing, it was confirmed that the CD51negl-KIR2DL4 had been constructed succes sfully. Conclusion The recombinant eukaryotic expression vector CD51negl-KIR2DLA was successfully constructed.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2002年第3期170-172,共3页
Immunological Journal
基金
国家自然科学基金资助项目(30070784)
