摘要
目的 :建立HPLC法测定肉苁蓉总苷胶囊中异类叶升麻苷含量。方法 :固定相为C1 8ODS柱 ;流动相为乙腈 - 1 .5 %乙酸溶液 ,其中乙腈线性梯度 1 1 .5 %～ 2 0 % (0～ 2 5min) ;检测波长 335nm。结果 :异类叶升麻苷的线性范围为 0 .2～1 .0 μg ,r=0 .9996 ,样品平均回收率为 98.85 % ,RSD =1 .51 % (n =9)。结论 :本法简便、准确、灵敏度高 ,重复性好 。
Objective:To establish a determination of acteoside isomer in Total Cistanchis Glycosides Capsules by HPLC.Methods: HPLC analysis was carried out on C 18 ODS column with acetonitrile——1.5% acetic acid as the mobile phase. The linear gradient of acetonitrile was from 11.5% to 20%(0-25min). The detection wavelength was 335nm. Results: The method was linear in the range of 0.2～1.0μg, r =0.9996. The average recovery was 98.85%, RSD was 1.51%( n =9). Conclusion:This method is simple, accurate, highly sensitive and reproducible. It may be used for the determination of acteoside isomer in Total Cistanchis Glycosides Capsules.
出处
《中成药》
CAS
CSCD
北大核心
2002年第5期352-354,共3页
Chinese Traditional Patent Medicine
