摘要
实验在培养的大鼠海马神经元中观察了重组人白细胞介素 6 (recombinanthumaninterleukin 6 ,rhIL 6 )对缺氧 复氧后Bcl 2、Bax表达和神经元凋亡的影响。把培养 12d的大鼠海马神经元分为对照组和rhIL 6组 ,同时于缺氧环境 (90 %N2 +10 %CO2 )中培养 2、4h后 ,再于常氧培养箱内复氧培养 2 4和 72h。于不同时间取出 ,分别用抗Bcl 2和Bax抗血清进行免疫组织化学染色 ,观察缺氧 复氧后大鼠海马培养神经元Bcl 2和Bax的表达 ,并用原位末端标记 (TUNEL)法和流式细胞术分别检测缺氧 复氧对体外培养海马神经元凋亡的影响。结果可见 ,与缺氧前相比 ,缺氧 复氧后 2 4和 72h ,海马神经元Bcl 2表达明显减弱 ,Bax表达明显增强 ,凋亡神经元明显增多。经rhIL 6预处理的海马神经元与对照组相比 ,缺氧 复氧后 2 4和 72h ,Bcl 2表达明显增强 ,Bax表达明显减弱 ,凋亡神经元明显减少。本实验结果提示 ,rhIL 6对海马神经元缺氧
The purpose of the present study was to determine the effects of recombinant human interleukin 6 (rhIL 6) on the Bcl 2 and Bax expression and apoptosis after anoxia reoxygenation in cultured rat hippocampal neurons. The control and rhIL 6 treated hippocampal neurons cultured for 12 d were exposed to anoxia environment (90% N 2+10% CO 2) for 2 and 4 h, and then were reoxygenated for 24 and 72 h. The expression of Bcl 2 and Bax was revealed immunocytochemically using the antiserum against Bcl 2 and Bax. The apoptosis was examined by the terminal deoxynucleotidyl transferase mediated biotinylated deoxyuridine triphosphate nickel end labeling (TUNEL) method and flow cytometric analysis. The results showed that in cultured hippocampal neurons the Bcl 2 expression decreased while Bax expression and the percentage of apoptotic neurons increased after anoxia reoxygenation compared with those before anoxia. In comparison with the control, after anoxia reoxygenation the Bcl 2 expression in hippocampal neurons was higher than that in rhIL 6 group; however, the Bax expression and the precentage of the apoptosis were decreased in rhIL 6 group. It is suggested that rhIL 6 may play a role in protecting neurons from the damage induced by anoxia reoxygenation.
出处
《生理学报》
CAS
CSCD
北大核心
2002年第2期115-120,共6页
Acta Physiologica Sinica
基金
ThisworkwassupportedbytheNationalNaturalScienceFoundationofChina (No 3 973 0 190 )
