摘要
为探讨砷和 5 -氮胞苷对人淋巴细胞 DNA损伤及修复的联合作用 ,应用单细胞凝胶电泳 (SCGE)技术比较研究了 5 -氮胞苷与砷同时和前后作用于人类淋巴细胞产生的联合毒性 ,结果显示 10 μmol/ L 5 -氮胞苷和 10 μmol/ L砷单独处理人淋巴细胞 2 h引起明显的 DNA泳动 (彗星尾 ) ,但两试剂引起的 DNA泳动 (彗星尾 )间无显著差异 ,5 -氮胞苷前处理与砷后处理 2 h引起的彗星尾与其单独处理组比较非常显著 ,砷前处理与 5 -氮胞苷后处理引起的彗星尾与其单独处理组比较无显著性差异 ,但较对照组差异显著。 10 μmol/ L 5 -氮胞苷和 10 μmol/ L砷分别单独处理 2 h引起了人淋巴细胞显著的 DNA损伤 (链断裂 ) ,5 -氮胞苷与砷在对淋巴细胞 DNA的损伤上表现为单纯相加作用。 5 -氮胞苷前处理显著增加了细胞对砷的基因毒性的敏感性 ,或砷后处理显著增加了 5 -氮胞苷引起的 DNA损伤 ,5 -氮胞苷后处理 2 h显著抑制了细胞对砷所致
In order to study the interaction of arsenic (As) and 5 azacytidine (5 azaC) in damaging DNA and inhibiting repair of human lymphocytes, single cell gel electrophoresis (SCGE) assay was used to study DNA damage in human lymphocytes treated in combination, by pre treatment or post treatment with 5 azaC and As for 2 hours at 37℃ and 5% CO 2. 10μmol/L 5 azaC or 10μmol/L As significantly induced tail moment in human lymphocytes, while the tail moment was no significance in the cells by combined treatment compared with treatment alone. Pre treatment of 5 azaC and post treatment of As significantly increased the tail moment compared with treatment alone. Induced tail moment by pre treatment of As and post treatment 5 azaC was not significant compared with treatment but alone significant compared with control. 5 azaC and As by combined treatment resulted in simple addition in cytotoxicity. 5 azaC, by pre treatment, increased the sensitivity of cells to the cytotoxicity of As, or As, by post treatment, significantly enhanced the 5 azaC induced DNA damage.5 azaC, by post treatment, arrested the DNA repair activity of the arsenic induced DNA damage.
出处
《地方病通报》
2002年第1期1-4,共4页
Endemic Diseases Bulletin
基金
国家留学基金委 2 0 0 0年度资助 (98952 0 1 0 )
