摘要
目的研究P53、Bcl-2和Bax蛋白在骨肉瘤中的表达情况,探讨P53、Bcl-2和Bax基因在骨肉瘤发生和发展中的作用,研究三者在骨肉瘤中的表达与骨肉瘤组织学分级之间的关系.方法采用S-P免疫组化方法观察42例骨肉瘤中P53蛋白、Bcl-2蛋白和Bax蛋白的表达情况.结果 1.在骨肉瘤中P53蛋白、Bcl-2蛋白和Bax蛋白的阳性率分别为61.9%、66.7%、61.9%.2.骨肉瘤Ⅰ～Ⅲ级中P53蛋白阳性率分别为41.7%、53.3%、85.7%,在Ⅰ级与Ⅲ级之间存在显著性差异(P＜0.05).3.骨肉瘤Ⅰ～Ⅲ级中Bcl-2蛋白阳性率分别为41.7%、62.5%、92.9%.在Ⅱ级与Ⅲ级之间存在显著性差异(P＜0.05),在Ⅰ级与Ⅲ级之间存在显著性差异(P＜0.01).Bax蛋白阳性率在各级间无显著性差异(P＞0.05).4.在骨肉瘤P53蛋白表达阴性组中Bcl-2蛋白的阳性率显著低于P53蛋白表达阳性组(P＜0.01).5.在骨肉瘤Bcl-2蛋白表达阴性组中Bax蛋白的阳性率显著低于Bcl-2蛋白表达阳性组(P＜0.05).结论利用S-P免疫组化法检测骨肉瘤中P53、Bcl-2和Bax蛋白的表达具有较高的敏感性及临床实用价值.
ve To study the expression of P53, Bcl-2 and Bax protein in osteosarcoma, and dis-cuss the function of P53,Bcl-2 and Bax gene in the occurrence and development of human osteosarcomas.to study the relation between the expression of the three proteins and the pathological classification in osteosarco-mas. Methods 42 cases of osteosarcoma have been examined with the method of S-P immunohistochemistry. Results 1.The expression rate of P53, Bcl-2 and Bax protein in osteosarcoma was respectively 61.9%, 66.7% and 61.9% .2.The expression rate of P53 protein in osteosarcoma grade Ⅲ was higher than that in osteosarcoma grade I (P<0.05).3.The expression rate of Bcl-2 protein in osteosarcoma grade Ⅲ was higher than that in osteosarcoma grade I (P<0.01) ,but the expression rate of Bax protein had no significant dif-ference(P>0.05).4.The expression rate of Bcl-2 protein in cases with negative P53 protein expression was lower than that in cases with positive P53 protein expression(P<0.01).5.The expression rate of Bax protein in cases with negative Bcl-2 protein expression was lower than that in cases with positive Bcl-2 protein expres-sion(P<0.05).Conclusion Expression of P53,Bcl-2 protein is related to the pathological classification and can reflect the malignant degree of osteosarcoma. S-P imrnunohistochernistry method has a higher sensitivity and clinical value.
出处
《中国骨伤》
CAS
2002年第4期214-216,共3页
China Journal of Orthopaedics and Traumatology
