摘要
对比MNC和CD34 +富集细胞在SCF +IL 3+IL 6 +FL +Tpo细胞因子组合下的体外扩增特性 ,发现 :CD34 +富集细胞具有很高的扩增潜力 ,在本实验条件下其总细胞持续扩增了 8周 ,扩增倍数达 312 70 9± 86 40 5倍 ;而MNC在培养至第 4周扩增就已呈现下降趋势 ,最大仅扩增了 5 3 3± 6 2倍。对比集落和CD34 +细胞的扩增发现 ,MNC的集落密度和CD34 +细胞含量由第 0天至第 7天有一个上升的过程 ,而CD34 +富集细胞在培养过程中 ,集落密度和CD34 +细胞含量却始终呈下降趋势。在体外培养过程中 ,CD34 +富集细胞的CFU GM和CD34 +细胞最大分别扩增了 185 7± 14 1和 191 7± 188 8倍 ,明显高于MNC的 12 4± 3 2和 5 0 6± 33 2倍 ;而CD34 +富集细胞和MNC的BFU E则只实现了少量扩增 ,分别为 7 2± 5 2和 10 1± 3 4倍。结果显示 ,从CD34 +富集细胞出发扩增造血干 祖细胞 ,可以得到更多的CD34 +细胞和CFU
For in vitro studies, both CD34 + selected cell and mononuclear cell (MNC) can be used to expand hematopoietic stem/progenitor cells. To investigate the expansion characteristics of mononuclear cells (MNC) and CD34 + selected cells the two cell fractions were cultured in the medium containing cytokine cocktails of SCF+IL-3+IL-6+FL+Tpo. It was found that the CD34 + selected cells had presented a high proliferation potential. The expansion of CD34 + selected cells could be maintained for 8 weeks while that of MNCs declined after 4 weeks. During the culture period,the maximum expansion of total cells in CD34 + selected cell culture achieved 31270.9±8640.5 times,while that of MNC reached 50.9±8.2 times only. In the culture of MNCs, the colony density and the proportion of CD34 + cells increased from day 0 to day 7. However,in the culture of CD34 + selected cells,both the colony density and the proportion of CD34 + cells declined continuously during the whole culture period. During the ex vivo culture of CD34 + selected cells, the maximum expansion of CFU-GM and CD34 +cells achieved 185.7±14.1 fold and 191.7±188.8 fold, respectively. They are much higher than that of MNC, which were 12.4±3.2 fold and 50.6±33.2 fold only. While the BFU-E of both cell fractions only expanded by few times, which were 7.2±5.2 and 10.1±3.4 times, respectively. The results showed that the CD34 + selected cells culture could obtain more CFU-GM cells and CD34 + cells during the whole culture period.
出处
《生物工程学报》
CAS
CSCD
北大核心
2002年第3期343-347,共5页
Chinese Journal of Biotechnology
基金
国家"8 6 3"计划生物领域项目
上海市现代生物与医药项目基金资助 (No.10 2 12 0 8 0 1
No .0 0 43190 0 3)~~
关键词
体外培养
脐血单个核细胞
CD34^+富集细胞
hematopoietic stem/progenitor cells, umbilical cord blood, MNC, CD34 + cell, long-term culture
