摘要
目的 :研究二甲基亚砜 (DMSO)对人肝癌细胞凋亡的诱导作用。方法 :用不同浓度的DMSO处理体外培养的人肝癌细胞BEL 74 0 2 ,应用普通光镜、荧光显微镜、MTT分析方法和流式细胞技术 (FCM )检测肝癌细胞凋亡的形态学变化、细胞存活率、凋亡百分率和细胞周期分布的变化。结果 :DMSO诱导BEL 74 0 2细胞核DNA凝缩和核片段化 ,最后形成凋亡小体 ;随着DMSO浓度的增加和处理时间的延长 ,细胞存活率明显下降 ,其IC50 为 1.9% ;2 %的DMSO处理细胞 12h ,凋亡率达 17.2 1% ,同时S期细胞明显增加 ,G2 M期细胞明显下降。结论 :DMSO可诱导人肝癌细胞凋亡 ,并使细胞受阻于S期而进入凋亡程序。
Objective: To study the apoptosis inducing function of dimethyl sulfoxide (DMSO) in human hepatocellular cancer cells BEL 7402. Methods: Light and fluorescence microscope, MTT assay and flow cytometry (FCM) were used to determine morphological changes, cell viability, apoptosis rate and cell cycle distribution. Results: DMSO induced the chromatin condensation, nuclear fragmentation and the formation of apoptotic body at the concentrations of 0.5% to 4%. Cell viability decreased with raised concentrations and prolonged treatment of DMSO. The IC 50 was 1.9% and apoptosis rate was 17.21% when the cells were treated with 2% DMSO for 12 h. At the same time, the number of cells in S phase increased while the number in G 2 M phase decreased. Conclusion: DMSO induced apoptosis and blocked the cell cycle at S phase in human hepatocellular cancer cells BEL 7402.
出处
《山东大学学报（医学版）》
CAS
2002年第2期103-105,共3页
Journal of Shandong University:Health Sciences
基金
教育部高校骨干教师科研基金资助课题
关键词
肝肿瘤
凋亡
二甲基亚砜
细胞周期
Hepatocellular cancer
Apoptosis
Dimethyl sulfoxide
Cell cycle
