摘要
采集石灰性土壤样品进行了溶磷微生物的筛选 ,获得了具有溶磷作用的草酸青霉菌菌株P8和Pn1。不同培养条件下测定了它们的溶磷能力 ,并与拜莱青霉菌ATCC2 0 85 1和解磷巨大芽孢杆菌ATCC14 5 81进行了比较。在固体培养基上草酸青霉菌P8、Pn1表现较强的溶解Ca3 (PO4) 2 、Ca8H2 (PO4) 65H2 O、CaHPO4、FePO4和骨粉的能力 ;在液体培养条件下 ,能有效的溶解摩洛哥磷矿粉 ,氮源对其溶磷效果有显著影响 ,硝态氮高于铵态氮 ;接种P8能够显著增加灭菌和不灭菌土壤的有效磷含量 ,灭菌土壤增加的有效磷略高于不灭菌土壤。氮源影响草酸青霉菌产生有机酸的种类 ,使用铵态氮时主要分泌苹果酸、乙酸、丙酸、柠檬酸、琥珀酸 ,而硝态氮条件下几乎不再产生这些有机酸。这表明 ,氮源形态影响了它的代谢方向 ,而且它的溶磷机理不只一种 ,其机理尚不清楚 。
Two Penicillium oxalicum strains P8 and Pn1 were isolated in Calcareous soils and their effects on solubilization of insoluble phosphate were tested under different conditions. In plate assay, P8 and Pn1 strains showed higher solubilizing capacity to Ca 3(PO 4) 2,Ca 8H 2(PO 4) 6 5H 2O,CaHPO 4,FePO 4 and bonemeal. In broth assay, P8 and Pn1 consistently demonstrated marked solubilizing efficiency of Morocco rock phosphate (MRP) compared to ATCC20581. Both NO 3 - N and NH 4 + N enhanced the release amount of P in MRP, but NO 3 - N form is better than NH 4 + N form. P8 strain could mobilize insoluble phosphate from soil, soil available P was increased significantly in both sterile and non sterile soils. The increment of available P in sterile soil was higher than that in non sterile soil. Nitrogen sources influenced acid metabolism of P8 and Pn1, strain P8 secreted malic acid, acetic acid, citric acid and propionic acid at the presence of NH 4 + N, but P8 produced none of the above organic acids with NO 3 - N supply, Pn1 showed the same trends. These indicated that there are more than one kind of mechanisms of P solubilization of Penicillium oxalicum P8.
出处
《中国农业科学》
CAS
CSCD
北大核心
2002年第5期525-530,共6页
Scientia Agricultura Sinica
