摘要
目的 研究pcDNA3.1·PTH治疗甲状旁腺功能低下症时PTH的变化及PTH与pcDNA3.1·PTH剂量的关系。方法 手术去掉家兔甲状旁腺 ,建立甲状旁腺功能低下症模型。将已构建的人甲状旁腺激素基因pcD NA3.1·PTH以 10 0 μg/ml、30 0 μg/ml、5 0 0 μg/ml剂量注射模型家兔的骨骼肌内 ,测定注射后不同时间的血液PTH。结果 注射pcDNA3.1·PTH后 2 4h模型家兔的PTH升高 ,7d达高峰水平 ,并持续 2个月 ,以 30 0 μg/ml和 5 0 0 μg/ml为佳。结论 人甲状旁腺激素基因治疗甲状旁腺功能低下是有效的 ,且以 30 0 μg/ml和 5 0 0
Objective Measuring the concentration of PTH when pcDNA3.1·PTH was injected into the muscle of rabbits with hypoparathyroidism (HPT),studying the relationship between change of blood PTH and pcDNA3.1·PTH dosage.Methods Different dosages of pcDNA3.1·PTH(100μg/ml, 300μg/ml and 500μg/ml)containing human PTH gene were injected respectively into muscle of the experimental rabbits after parathyroidectomy in advance.Then the concentration of PTH in blood of rabbits was measured at following different times.Results PTH level began to increase from the first day,which reached in peak concentration at the seventh day after the injection of pcDNA3.1·PTH and persisting two months.Conclusion Human PTH gene was effective in therapying HPT rabbits,their best effective dosages were 300μg/ml and 500μg/ml respectively.
出处
《中国医师杂志》
CAS
2002年第3期229-230,233,共3页
Journal of Chinese Physician
基金
黑龙江省教育厅基金资助项目(课题号:95510 6 4)
