摘要
从棉花花瓣cDNA文库中随机挑选部分克隆 ,经测序发现一个与拟南芥耐盐锌指蛋白基因同源的cDNA(CSTZ) .CSTZ序列全长 10 12bp ,开放阅读框共编码 2 72个氨基酸 ,含典型的植物双锌指 (Cys2 /His2 )结构区 .Northern杂交证实 ,CSTZ的表达随棉花幼苗钠盐处理浓度的升高而增强 .在棉花花龄期 ,CSTZ基因在叶片、根、花瓣和花药组织中大量表达 。
By sequencing analysis of the clones chosen from the cotton petal cDNA library, a cDNA, homologous to Arabidopsis salt tolerance zinc finger protein, was isolated and designated as CSTZ. This new cDNA (1 012 bp) contains an open reading frame of 819 bp encoding a 272 residue polypeptide that includes a typical plant two fingered Cys2/His2 zinc finger mitif. Nortern blot analysis revealed that the expressed level of CSTZ in cotton seedling increased in response to 220 mmol/L NaCl treatment. CSTZ is highly expressed in leaves, roots, petals, and anthers, but with low expression in stigmas.
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
2002年第1期42-46,共5页
Journal of Fudan University：Natural Science
关键词
棉花
耐盐
锌指蛋白
基因表达
cotton
salt tolerance
zinc finger protein
gene expression
