摘要
考察了流速、进样体积与柱床高度对人表皮生长因子 (humanepidermalgrowthfactor,hEGF)分离行为的影响 .结果表明 :在实验范围内 ,流速对hEGF的分配系数影响较小 ,hEGF的分配系数随样品体积的增加而上升 ,但柱床高度对hEGF的分配系数几乎无影响 .在流速小于 6cm·h-1时 ,hEGF的标准偏差下降显著 ,而高流速对其影响较小 .当进样量小于 3ml时 ,hEGF的标准偏差上升较缓 ;当进样量大于 3ml时 ,hEGF的标准偏差显著上升 .在柱床高度小于 8cm时 ,hEGF的标准偏差下降较快 ;当柱床高度高于 8cm时 。
Human epidermal growth factor (hEGF), a polypeptide growth factor comprising of 53 amino acid residues, has become one of research hot spots in medical field because of its various biological functions. In order to purify hEGF, the influences of flow rate, sample volume and column height on separation performance of hEGF in gel chromatography are investigated. Results indicate that there is a little influence of flow rate on distribution coefficient of hEGF and distribution coefficient of hEGF apparently increases with the increase of sample volume injected, while column height has no influence on distribution coefficient of hEGF. The standard deviation of hEGF decreases with the increase of flow rate and column height, especially when flow rate is less than 6 cm·h-1 and column height is lower than 8 cm. It also increases with the increase of sample volume injected, especially when the sample volume injected is larger than 3 ml. These results imply that the resolution will sharply decrease when flow rate and column bed height are less than 6 cm·h-1 and 8 cm respectively and the sample volume injected is more than 3 ml under given conditions.
出处
《化工学报》
EI
CAS
CSCD
北大核心
2002年第4期369-372,共4页
CIESC Journal
基金
国家自然科学基金资助项目 (No .2 973 6180 )~~
关键词
人表皮生长因子
多肽
凝胶过滤
层析
生化分离
Amino acids
Biochemistry
Biomedical engineering
Gel permeation chromatography
Polypeptides
Separation
