摘要
目的 在染色体 8p2 1 8p2 2界定发生于中国人肺腺癌的杂合性缺失 (LOH)的最小区域 ,为定位克隆肺腺癌相关抑癌基因提供线索。方法 利用 32例肺腺癌患者肿瘤组织检测位于 8p2 1～p2 2的 17个微卫星多态性标记的LOH频率 ,并且探讨各位点LOH与病理分级和临床分期的关系。结果 在 32例肺腺癌患者组织中有 31例 (96 6 7% )存在至少 1个位点的LOH ;主要集中于 3个区域 :位于 8p2 2的D8S2 5 4～ 2 6 1、D8S182 7～ 1731以及D8S1135。所检测位点中仅D8S2 6 1位点的LOH发生频率与肺腺癌分期呈正相关 (P <0 0 5 )。结论 8p2 2区域可能存在位于D8S2 5 4～ 2 6 1,D8S1135和D8S182 7～ 1731的。
Objective To determine the common deletion region of loss of heterozygosity (LOH) on chromosome 8p21 8p22 in adenocarcinomas of lung, and facilitate identification of candidate tumor suppressor genes associated with adenocarcinoma of lung. Methods PCR and microsatellite analysis were used to examine the LOH frequency of 17 microsatellite loci at the 8p21 8p22 in the samples resected from 32 patients with lung adenocarcinoma and adenosquamous carcinoma. The relationship of LOH for each marker to pathological grade and that to clinical stage are investigated. Results Thirty one out of the 32 (96 67%) samples showed allelic loss in at least one of the 17 markers. The most frequent LOH loci were mainly located in the three regions: D8S254 261, D8S1827 1731, and D8S1135 loci. Among them, the LOH frequency of D8S261 locus was related to the stage of tumor ( P <0 05). Conclusion An interval of common deletion on chromosome 8p22, encompassing D8S254 261, D8S1827 1731 and D8S1135, might harbor candidate tumor suppressor gene(s) associated with pathogenesis of adenocarcinoma of lung.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2002年第11期740-742,共3页
National Medical Journal of China
基金
国家重点基础研究发展规划资助项目 (G19980 5 12 0 7)
