摘要
目的 探讨 β catenin、p5 3和增殖细胞核抗原 (PCNA)在大肠腺瘤癌变中的可能作用。方法 以免疫组织化学方法检测 77例大肠腺瘤 (CRA)及癌变组织中 β catenin、p5 3蛋白及PCNA的表达情况。结果 CRA随上皮不典型增生程度的提高以及癌变的出现 ,β catenin异常表达率逐渐增高(P <0 .0 1)。伴中、重度上皮不典型增生的CRA和CRA癌变组织中 ,β catenin核表达阳性率均明显高于上皮轻度不典型增生的CRA(P <0 .0 1)。CRA伴轻度不典型增生和CRA伴中、重度不典型增生及CRA癌变 3组的p5 3蛋白和PCNA表达依次增高 ,其阳性率分别为 10 .3%、43.8%、75 .0 %和 17.2 %、6 2 .5 %、87.5 %。在 β catenin核表达阳性者中 ,其PCNA的强阳性表达率明显高于 β catenin核表达阴性者 (6 9.7%和 36 .4% ,P <0 .0 5 ) ;在p5 3蛋白表达阳性者中 ,其PCNA的强阳性表达率明显高于p5 3表达阴性者 (72 .4%和 37.5 % ,P <0 .0 5 )。在CRA癌变组中 ,有 5 0 .0 %可见 β catenin和p5 3同时表达。结论 β catenin、p5 3和PCNA在CRA癌变过程中可能发挥了重要作用。
Objective Research was done on the possible roles of β-catenin, p53 and proliferating cell nuclear antigen (PCNA) in the carcinogenesis of colorectal adenoma (CRA). Methods β-catenin and p53 and PCNA expressions were studied with immunohistochemical stain in 77 specimens of CRA together with mild epithelial dysplasia (CRA-MD), CRA with moderate/severe epithelial dysplasia (CRA-D/SD) and CRA with cancerous changes (CRA-C). Results The percentage of abnormal expression of β-catenin increased during the transition from CRA-MD to CRA-D/SD to CRA-C ( P <0.01). The nuclear expressions of β-catenin in CRA-D/SD and CRA-C were all significantly higher than that in CRA-MD. Expression of p53 and PCNA were increased from CRA-MD to CRA-D/SD to CRA-C, with the positive rates in these three groups of 10.3%, 43.8%, 75.0% for p53 and 17.2%, 62.5%, 87.5% for PCNA, respectively. 69.7% of cases with positive nuclear β-catenin expression showed strong PCNA positivity which was much higher than the 36.4% of cases without nuclear β-catenin expression ( P <0.05). The percentage of strong PCNA expression in the p53 positive cases was significant higher than that in cases with negative p53 expression (72.4% vs 37.5%, P <0.05). Nuclear β-catenin and p53 co-expression rates in CRA-C reached 50%. Conclusion β-catenin, p53 and PCNA may play important roles in the carcinogenesis of colorectal adenoma.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2002年第3期264-267,共4页
Chinese Journal of Oncology
