摘要
Gelatin was covalently immobilized onto the polyurethane membrane surface grafted previously with poly(methacrylic acid) (PU g PMAA) using 1 [(3 dimethylamino)propyl] 3 ethyl carbodiimide as the condensing reagent. ATR FTIR and ESCA characterizations verify the occurrence of the grafting, and prove that the immobilized amount of gelatin is proportional to the number of active sites, i.e . carboxylic groups on PU surface. Endothelial cells culture reveals that the cell attachment and cell growth on PU g PMAA g Gelatin are both improved obviously, demonstrating that a cell compatible PU is obtained and the immobilizing method is quite effective to introduce cell growth factors onto polymer surface.
Gelatin was covalently immobilized onto the polyurethane membrane surface grafted previously with poly(methacrylic acid) (PU g PMAA) using 1 [(3 dimethylamino)propyl] 3 ethyl carbodiimide as the condensing reagent. ATR FTIR and ESCA characterizations verify the occurrence of the grafting, and prove that the immobilized amount of gelatin is proportional to the number of active sites, i.e . carboxylic groups on PU surface. Endothelial cells culture reveals that the cell attachment and cell growth on PU g PMAA g Gelatin are both improved obviously, demonstrating that a cell compatible PU is obtained and the immobilizing method is quite effective to introduce cell growth factors onto polymer surface.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2002年第6期1210-1212,共3页
Chemical Journal of Chinese Universities
基金
国家自然科学基金 (批准号 :5 980 3 0 0 6)
国家重点基础研究发展规划项目 (批准号 :G19990 5 43 0 5 )资助
