摘要
目的 探索一种简便易行并能同时显示大鼠中枢神经系统 (CNS)神经元和神经纤维的全程连续切片方法。 方法 经过灌流固定的CNS组织低温冰箱速冻后连续冰冻切片和改良的苏木精染色方法。 结果 大鼠全程CNS连续切片各断面上神经元呈蓝黑色 ,神经纤维呈蓝色 ,背景淡黄色。 结论 运用低温速冻、连续冰冻切片、改良的苏木精染色方法可制作同时显示CNS神经元和神经纤维的全程连续切片。
Objective To explore a practicable method of serial sections of whole CNS in the rat, in which both neurons and nerve fibers were showed at the same section. Methods After perfused fixation and quick freezing, the tissues of whole CNS were cut into serial sections with cryostat followed by hematoxylin stain. Results The nerve fibers were stained in blue color while the neurons were showed black-blue and the background was light-yellow.Conclusion Our method was feasible to show both neurons and nerve fibers at the same section with quick freezing, freezing serial sectioning and improved hematoxylin staining.
出处
《解剖学报》
CAS
CSCD
北大核心
2002年第3期317-320,共4页
Acta Anatomica Sinica
基金
第三军医大学校教学基金资助项目 ( 2 0 0 10 6 )
关键词
中枢神经系统
神经纤维
神经元
连续冰冻切片
苏木精染色
大鼠
Central nervous system
Nerve fiber
Neuron
Cryostat serial sectioning
Improved hematoxylin staining
Rat
