摘要
白腐真菌黄孢原毛平革菌产生的胞外过氧化物酶系由两类同功酶木素过氧化物酶和锰过氧化物酶组成。木素过氧化物酶对所研究的染料都有降解作用 ,锰过氧化物酶只对部分染料起作用。加入 H2 O2 ,胞外酶液可以降解多种酸性染料如卡布龙红、酸性大兰、弱酸黄和弱酸大红 ,它们的脱色速率依次下降。以卡布龙红为例研究了各种条件因素对其降解脱色的影响 ,最佳 H2 O2 浓度是 10 0μmol/L,最佳 p H为 4.0 ,温度越高 ,脱色越快 ,但高于 5 0℃酶将明显失活 ,木素过氧化物酶活性越高脱色速率越快 ,高浓度染料对其脱色有抑制作用。最佳条件下 ,5 0 mg/L 浓度卡布龙红第一分钟的脱色率达 70 %。随着稀释倍数的增加 ,酶液的降解速率呈抛物线加速下降 。
The extracellular ligninolytic peroxidases from the white rot fungus phanerochaete chrysosporium were composed of two coenzymes namely lignin peroxidases and Mn dependent peroxidases. Lip enhanced the degradation of all studied azo dyes whereas Mnp could be effective for one dye. After adding H 2O 2, the culture supernatant with Lip and Mnp could decolor many acidic dyes such as Carplon red, acidic bright blue, week acidic yellow and week acidic bright red, their decoloring rate decreased in turn. In decoloring Carplon red, the optimal H 2O 2 concentration was 100 μmol/L, the optimal pH is 4.0. Higher Lip activity increased the decoloring rate and so do temperature until 40 ℃ which deactivated the enzyme. High concentration of dyes inhibited the decoloration. In the optimal condition, percent of decoloration of Carplon red at the concentration 50 mg/L by the culture supernatant was 70%. When increase of extent of supernatant dilution, the decoloring rate decreased accelerately. Some components in the supernatant such as some metal ions might be important for enzyme activity.
出处
《环境污染与防治》
CAS
CSCD
北大核心
2002年第2期71-75,共5页
Environmental Pollution & Control
基金
国家自然科学基金资助项目 (No.2 99760 3 8)
