摘要
观察补肾煎对去势兔动脉粥样硬化血管平滑肌细胞增殖和凋亡的影响及机制。将 2 7只纯种新西兰雌兔随机分为 :A组 (卵巢切除 )、B组 (未切除卵巢 )、C组 (卵巢切除 +补肾煎 ) ,均给予高脂饮食 ,同时注射牛血清白蛋白 0 2 5g/kg ,造成动脉粥样硬化模型 ,12周时比较动脉形态学变化、MTT法测定各组培养血管平滑肌细胞增殖活性、电镜观察、原位末端标记法及流式细胞仪了解各组平滑肌细胞凋亡情况。结果 :C组兔主动脉内膜增生低于A组 ;C组培养平滑肌细胞增殖低于A组及B组 ;C组培养平滑肌细胞凋亡高于A组及B组。结论 :补肾煎可能通过抑制平滑肌细胞的增殖及增加其凋亡而减轻去势兔动脉粥样硬化的形成。
To investigate the effects and mechanism of 'Kidney-Nourishing Decoction' on proliferation and apoptosis of vascular smooth muscle cells (VSMC) in ovariectomized rabbits,27 female New Zealand rabbits were randomized into three groups:group A(OVX+cholesterol feeding+bovine serum albumin),group B(sham operation+cholesterol feeding+bovine serum albumin)and group C(OVX+cholesterol feeding+bovine serum albumin+'Kidney-Nourishing Decoction').After fed for 12 weeks,the rabbits were sacrificed and the proliferative activity and apoptosis of VSMC were observed.Results:The aortic tunica intima proliferated less in group C than in groups A and B;the VSMC proliferated less in group C than in groups A and B;but the apoptosis was more significant in group C than in groups A and B.This suggests that 'Kidney-Nourishing Decoction' may inhibit the proliferation of VMSC and promote its apoptosis,as a consequence,to relieve atherosclerosis.
出处
《上海中医药杂志》
北大核心
2002年第6期6-8,共3页
Shanghai Journal of Traditional Chinese Medicine
基金
上海市卫生局中医特色专科基金资助项目 ( 2 0 0 0 -4 7)
关键词
补肾煎
动脉粥样硬化
平滑肌细胞
细胞增殖
细胞凋亡
绝经
A3
药理学
Kidney-Nourishing Decoction'
ovariectomized rabbit
atherosclerosis
smooth muscle cell
proliferation
apoptosis
