摘要
目的 通过大鼠糖尿病模型 ,观察抗氧化剂对糖尿病大鼠肾小球蛋白激酶的影响。方法 将 75只雄性Wistar大鼠分为正常对照组、糖尿病未治疗组、抗氧化剂治疗组各 2 5只 ,共观察 8周 ,分别测定尿白蛋白排泄量(UAE) ,内生肌酣消除率 (Ccr)、血浆及肾脏组织一氧化氮 (NO)、一氧化氮合成酶 (NOS)、内皮素 (ET)和肾小球蛋白激酶C(proteinkinaseC ,PKC)。结果 给予维生素E治疗组 8周时 ,Ccr[(5 .2 8± 0 .5 4)ml/(min·kg) ]及尿白蛋白排泄量 [(14.2 7± 1.16 ) μg/2 4h]显著低于未治疗组 ,肾小球细胞膜PKC[(6 8.2 7± 12 .33) pmol/(min·mgprotein) ],2周时N0 [(34 .2 3± 3.91) μmol/L]及NOS[(32 .0 7± 3.76 )U/L]明显低于未治疗组 ,维生素E治疗组 2周时与 8周时的NO及NOS下降幅度明显小于未治疗组。结论 维生素E具有抑制PKC活性作用。
Objective To investigate the effect of vitamin E on reduction of PKC activity in streptozotocine induced diabetic rats.Methods Vitamin E 20 mg/kg/d was given to diabetic rats for 8 weeks. Urinary albumin excretion (UAE) was measured by radioimmunoassay. The changes of creatinine clearence rate (Ccr), nitric oxide (NO), nitric oxide synthase (NOS) and endothelin (ET) in plasma and renal cortical tissue, membrane protein kinase C (PKC) in renal glomeruli were observed.Results After the treatment of vitamin C, Ccr (4.61±0.12 ml/min/kg), UAE (17.40±1.22 μg/24 h), PKC (60.21±9.78 pmol/min/mg protein) at 8th week decreased significantly. Both NO(30.23±2.01 μmol/L) and NOS (35.21±1.54 U/L) in plasma and kidney tissue were lowered as compared with untreated diabetic group in second week. Vitamin E also delayed in lowering NO and NOS levels in 8 weeks.Conclusion Vitamin E can reduce PKC activity in diabetic rats.
出处
《同济大学学报（医学版）》
CAS
2002年第2期90-92,共3页
Journal of Tongji University(Medical Science)
基金
铁道部医学专项基金资助项目 (TW 2 0 0 2 30 )
