摘要
目的 建立新的帕金森病 (Parkinson’sdisease ,PD)动物模型 ,探讨其发病机制。方法 在大鼠脑黑质(substantianigra ,SN)内注射脂多糖 (Lipopolysaccharide ,LPS)后 ,按大鼠不同存活期用高效液相色谱 (HPLC)来测定脑内多巴胺 (Dopamine,DA)及其代谢产物的含量 ;用免疫组化法观察酪氨酸羟化酶 (Tyrosinehydroxylase ,TH)阳性神经细胞、小胶质细胞的形态及数量变化。结果 DA及其代谢产物的含量在LPS注射侧随时间不同有不同程度下降 ,于第 14天达到最低 (P <0 0 1) ;注射侧黑质TH阳性神经元可以达到全部消失 ,该处可见大量被激活并有形态改变的小胶质细胞。
Objective To creat a new rodent model of Parkinson's disease (PD) for the investigation of its pathological mechanism. Methods At different survival time after injection of lipopolysaccharide(LPS) in the substantia nigra (SN) of rats, the levels of DA and their metabolites were measured by using HPLC, and the tyrosine hydroxylase (TH) positive neurons and microglia were observed by immunohistochemistry. Results The levels of DA and their metabolites were decreased and the lowest level ( p <0 01) appeared at 14th day after injection of LPS. The number of TH positive neurons was also reduced from the second day after injection, and a large amount of microglial cells were activated with their shapes changed. Conclusions The injected LPS could result in a damage of dopaminergic neurons in the SN.
出处
《中国实验动物学报》
CAS
CSCD
2002年第2期81-85,共5页
Acta Laboratorium Animalis Scientia Sinica
基金
北京市资助项目 (95 40 2 43 0 0 )
