摘要
Dynamin, a 100-kD GTPase first found in animal cells, is essential for vesicle formation in receptor-mediated endocytosis, synaptic vesicle recycling, and possibly vesicle trafficking in and out of the Golgi apparatus. Recently, dynamin-like proteins were also found in some plant cells. We demonstrate here the presence of dynamin with molecular weight of 100 kD in day-lily ( Hemerocallis fulva) pollen based on molecular estimation and Western blotting. The highly purified pollen Dynamin had GTPase activity, which could be stimulated 1.64 fold by calf brain microtubules in vitro. The results from electron microscopic examination showed that the pollen dynamin readily self-assembled into ring-like structures.
动力蛋白 (dynamin)是一类具有可被微管激活的GTP酶活性的新型马达蛋白 ,被证明在动物细胞受体介导的内吞小泡的形成、突触小泡再循环及高尔基体的囊泡运输中起关键作用。近几年 ,一些植物细胞也被发现有动力蛋白类似物。本研究通过分子量鉴定和免疫印迹法证明萱草 (HemerocallisfulvaL .)花粉中存在动力蛋白 ,其分子量为 1 0 0kD ;经过高度纯化的花粉动力蛋白仍具有GTPase活性 ,且可被牛脑微管激活 1 .6 4倍 ;电子显微镜观察结果表明 。
基金
国家自然科学基金 ( 39730 2 80 )~~
