摘要
目的 观察芒果甙对人肝癌细胞株BEL 74 0 4的毒性和诱导凋亡作用及对细胞周期的阻滞作用 ,探讨芒果甙作为肿瘤化学预防药物的可能性。方法 用MTT法观察芒果甙对人肝癌细胞株增殖的抑制作用 ,用光学显微镜观察芒果甙对细胞的毒性作用 ,以流式细胞仪检测芒果甙对细胞凋亡的诱导作用和对细胞周期的干预作用。结果 不同浓度的芒果甙在不同时间对肝癌细胞株均有毒性作用 ,并随浓度升高和作用时间的延长而毒性作用增强 ,凋亡也随之增加 ,芒果甙阻滞肝癌细胞周期于G2 /M期 ,2 0 μmol/L芒果甙作用 2 4h后上述效果开始明显。 结论 芒果甙对肝癌细胞株有明显的毒性作用 ,能诱导肝癌细胞凋亡和阻滞细胞周期于G2 /M期 ,具有潜在药用价值 。
Objective To observe the toxicity, apoptosis and cell proliferation cycle of Mangiferin on the BEL 7404 human hepatocellular carcinoma cell line, and explore the possibility of Mangiferin as a new tumor chemopreventive drug. Methods MTT method was used to determine the effect of Mangiferin on cell proliferation, microscopy method was used to observe cytotoxicity of Mangiferin, and flow cytometry method was used to determine apoptosis induction and cell cycle proliferation blocking effects of Mangiferin. Results The cytotoxicity effects were observed in various concentrations of Mangiferin and at different exposure times. The effects were enhanced as the Mangiferin concentration increased and exposure time prolonged. The apoptosis effect was also enhanced, and cell cycle arrested at G 2/ M phase. All the above effects became significant when cells exposed to the concentration from 20 μmol/L to 200 μmol/L of Mangiferin at 24 h. Conclusions Mangiferin is cytotoxic, and may induce apoptosis. Mangiferin has cell cycle blocking effects on hepatocellular carcinoma cells. Its pharmaceutical potentials is worth for further investigating.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2002年第6期341-343,共3页
Chinese Journal of Digestion
基金
广西自然科学基金资助项目 (0 0 70 3 7)
