摘要
目的观察海马、边缘区及相关脑区的基因表达谱是否存在差异,期望寻找到海马、边缘区的特异基因。方法从引产胎儿胎脑中准确取出海马、杏仁核、边缘区和尾壳核。用mRNA差异显示技术分析上述脑区的基因表达差异,并对海马的一个差异片段进行了克隆、测序。结果4个脑区相比,海马、边缘区各发现2个差异片段,海马的一个差异片段长度为277 bp,序列与编号为AC0049人类克隆序列完全同源。结论海马、边缘区等不同脑区间基因表达存在差异。
Objective To investigate the difference in the expression profiles in the hippocampus, marginal division and other related brain areas for identification of genes specific to the hippocampus and marginal division. Methods Fetal hippocampus, marginal division, caudate putamen and amygdala were obtained and mRNA differential display technique was employed to study the gene expression in the 4 brain areas. Results Two differentially expressed fragments were isolated from each of the hippocampus and marginal division, and cloning and sequence analysis of one of the fragment 277 bp in length from the hippocampus showed total homology with the sequence of human clone AC0049. Conclusion The 4 brain areas have different gene expression profiles.
出处
《第一军医大学学报》
CSCD
北大核心
2002年第6期503-505,共3页
Journal of First Military Medical University
基金
广东省自然科学基金重点项目(13069)
关键词
海马
边缘区
差异显示
聚合酶反应
杏仁核
尾壳核
differential display
polymerase chain reaction
hippocampus
marginal division
caudate putamen
amygdala
