摘要
为探讨筋膜瓣是否具有促组织工程骨修复羊负重骨大段骨缺损及其体内再血管化的作用 ,将 2 7只中国青山羊分为 3组 :单纯材料组 (珊瑚羟基磷灰石 ,CHAP)、组织工程骨组 (CHAP +经诱导分化的骨髓基质干细胞BMSc)、筋膜瓣组 (筋膜包裹CHAP +经诱导分化的BMSc)。制备单侧胫骨中段 2 0mm的骨膜与骨缺损 ,术后 2、4、8周行放射性核素骨显像 (ECT)检查 ,4、8、12周X线检查、组织学检查。结果显示 :术后修复骨缺损的能力和再血管化的程度由高到低依次为筋膜瓣组、组织工程骨组、单纯材料组。说明筋膜瓣对组织工程骨体内的再血管化过程有明显的促进作用。
To study the ability to repair the large segment defect of weight bearing bone of caprine with tissue engineered bone and the effect of revascularization of tissue engineered bone with fascia flap in vivo. 27 caprines were divided into three groups: group A (the defect was filled with coral hydroxyapatite CHAP); group B (the defect was filled with CHAP + bone marrow stroma cell BMSc); group C (the defect was filled with CHAP +BMSc + fascia flap). After BMSc was cultured and induced, CHAP in group B and C was combined with it. A 20mm bone defect was made and fixed with 93mm AO dynamic compression plate of levo tibia, and implanted with different materials as described above. Radionuclide bone imaging was used to monitor the revascularization of the implants 2, 4, 8 weeks after operation. X ray examination, optical density index of X ray film, HE staining of the implants were performed 4, 8, 12 weeks after operation to evaluate the bone regeneration and bone defect repairing effect. VG staining was an ideal method to judge the revascularization of implants. Group A showed some trace of bone regeneration. Group B manifested a good ability to repair the defect. In group C, with the assistance of fascia flap which gives more chance to make implants get more nutrient, the ability to repair the defect was quite strong. Fascia flap can accelerate the revascularization process of tissue engineered bone, and by this way, it augments the ability of tissue engineered bone to repair the large bone defect of caprine.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2002年第6期482-484,共3页
Medical Journal of Chinese People's Liberation Army
基金
国家重点基础研究发展规划 (编号G1 9990 543 0 9)
全军医学科研"十五"计划重大项目 (编号 0 1Z0 4 5)
广东省自然科学基金 (编号 990 4 1 4 )
广东省团队项目 (编号 1 0 71 71 41A1 2J1 5)资助课题
