摘要
为了探讨 HBV DNA、 c- fos和 p5 3在肝癌发生中的作用及其关系。利用 PCR技术和免疫组化 ABC法 ,检测了肝癌基因组中 HBV DNA的整合、 c- fos和突变 p5 3的表达。 HBV DNA整合率为 6 7% ,C- FOS蛋白阳性率为 6 7% ,突变 P5 3蛋白阳性率为 5 8%。 HBV DNA整合与 c- fos激活、 p5 3突变有显著的一致性 (P>0 .0 5 ;P>0 .0 5 ) ,c- fos激活与 p5 3突变之间呈负相关 ,但无显著意义 (r=- 0 .2 816 ,P>0 .0 5 )。 HBV DNA的整合可能引起 c- fos的激活和 /或 p5 3的突变 。
To investigate the actions HBV DNA, c fos and p53 genes in the occurrence of liver cacinoma and the relations among them. HBV DNA integration and expressions of c fos and mutant p53 genes in liver cacinoma genomes were detected with PCR and immunohistochemical ABC method. The rate of HBV DNA integration is 67%, the positive rates of c fos protein and mutant p53 protein are 67% and 58% respectively. There is signficant identity between HBV DNA integration and c fos activation, p53 mutation ( P >0 05; P >0 05). There is negative correlation between c fos activation and p53 mutation, but there is no significant ( r =-0 2816, P >0 05). HBV DNA integration might cause c fos activation and/or p53 mutation, and then lead to the occurrence of liver cacinama.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2001年第1期58-61,共4页
Chinese Journal of Histochemistry and Cytochemistry
