摘要
目的探讨套接周围神经缺损的最佳特异性再生间隙长度。方法50只大鼠平分5组,采用聚乳酸管套接双侧股神经,各组间隙为0mm、2mm、5mm、8mm和12mm。12周后,采用HRP逆行示踪,组内一侧股四头肌支和对侧隐神经支之间比较标记的运动神经元或感觉神经元数量。再生神经作光镜、电镜切片。结果5mm和2mm组中运动支标记的运动神经元和感觉支标记的感觉神经元数量最大,切片显示神经再生良好。结论聚乳酸管套接股神经缺损存在运动、感觉纤维特异性再生现象,其有效间隙长度为2～5mm,以5mm长度为优。
Objective To seek the best gap length of peripheral nerve tublization. Methods The Femoral nerve gaps of fifty rats in five groups were 0mm, 2mm, 5mm, 8mm and 12mm respectively, and all bridged with biodegradable polylactic acid (PLA) tubes. After twelve weeks,HRP(1mg) were applied to one motor branch and opposite sensory branch and compared the labeled motor or sensory neuron counts between motor and sensory branch. The regenerated nerves were excised for light and electron microscopy. Results In 2mm and 5mm groups, the number of labeled motor neurons and labeled sensory neurons was the most, nerve regeneration was good by light microscopy and electron microscopy. Conclusions For peripheral nerve tubulization, there are preferential reinnervation of regenerating motor and sensory fibres. The effective gap length is 2～5mm. The group of 5mm is the best.
出处
《中华创伤骨科杂志》
CAS
CSCD
2002年第1期45-48,共4页
Chinese Journal of Orthopaedic Trauma
基金
国家自然科学基金资助项目(39600149)
全军九五医药卫生科研基金资助项目(96Q052)
