摘要
目的 :研究乙酰胆碱 (ACh)诱发犬急性心房颤动模型的效果及机制。方法 :取 10条健康杂种犬 ,给予持续静注 ACh及心房快速起搏 ,测定房颤持续时间 ,根据房颤持续时间逐渐增加药量 ,直至通过心房快速起搏可以诱发出持续性房颤 (≥ 3m in)为止。给药前及给药后测定心率 ,校正窦房结恢复时间 ,心房有效不应期 ,给药前后及房颤发生后测定左室内压及左室内压最大上升、下降速率 (± dp/ dtmax)。结果 :在给予 ACh前 ,通过心房快速起搏均未能诱发出持续性房颤 ,随着 ACh量的逐渐增加 ,诱发出房颤的时间逐渐延长 ,当增加到一定量 (18± 8μg· kg- 1·min- 1 )之后 ,10只犬均可以通过心房快速起搏诱发出持续性房颤 ,有 1只犬不需诱发即可出现 ,其持续时间为 34 4± 173s。测定犬心率明显减慢 (15 7± 2 5 vs138± 2 0· m in- 1 ,P<0 .0 1) ,校正窦房结恢复时间延长 (4 4± 13vs81±2 4ms,P<0 .0 1) ,心房有效不应期明显缩短 (80± 16 vs 6 0± 13m s,P<0 .0 1) ,使用 ACh后左室内压及其最大上升、下降速率差异不显著 (140± 2 9vs 12 9± 15 m m Hg· s- 1 ,P>0 .0 5 ;2 75 2± 876 vs 2 434± 5 31mm Hg· s- 1 ;-3115± 10 6 7vs- 2 70 7± 714m m Hg· s- 1 ,P>0 .0 5 ) ,房颤发生后则明显降低 (12 9± 15
AIM:To investigate the effect and mechanism of acetylcholine(ACh) in inducing canine acute atrial fibrillation(AF) model. METHODS:It was performed on 10 mongrel dogs. In this study,intravenous(IV) infusion of ACh and atrial burst-pacing were performed on the dogs,then the dose of ACh was increased gradually according to the duration of AF until sustained AF(lasting≥3 min) could be induced.Sinus rate,corrected sinus-atrial recovery time(CSANRT) and atrial effective refractive period(ERP) were measured before and after ACh,and pressure of left ventricle, maximal increasing and decreasing velocity of left ventricle's pressure were also measured before and after ACh and after AF was induced. RESULTS: Before the ACh,sustained AF was failed to be induced by atrial burst-pacing. With the increasing of the dose of ACh, the duration of AF prolonged. When the ACh increasing to a certain dose(18±8 μg·kg -1·min -1), sustained AF could be induced in all ten dogs by atrial burst-pacing and AF occurred spontaneously in one dog. The mean duration was 344±173 s. Sinus rate droped markedly(157±25 vs 138±20·min -1, P<0.01), corrected sinus-atrial recovery time( CSANRT) was prolonged(44±13 vs 81±24 ms, P<0.01) and atrial ERP was shortened(80±16 vs 60±13 ms, P<0.01). Pressure of left ventricle, maximal increasing and decreasing velocity of left ventricle's pressure had no significant differences before and after ACh(140±29 vs 129±15 mm Hg·s -1, P>0.05;2752±876 vs 2434±531 mm Hg·s -1; -3115±1067 vs -2707±714 mm Hg·s -1, P>0.05),but decreased significantly after AF was induced(129±15 vs 79±14 mm Hg,P<0.05;2434±531 vs 1636±417 mm Hg·s -1, -2707±714 vs -1318±237 mm Hg·s -1, P<0.05). CONCLUSION:The model is highly reproducible,and sustained AF was readily and safely inducible for a prolonged period,which can partly imitate the physiopathological procedure of clinical AF.
出处
《心脏杂志》
CAS
2002年第3期195-198,共4页
Chinese Heart Journal
关键词
乙酰胆碱
心房快速起搏
心房颤动
动物模型
狗
acetylcholine
atrial burst-pacing
atrial fibrillation
animal model
dogs
