摘要
应用单向SDS-PAGE和双向IEF-SDS电泳技术对两个品种的普通小麦(T.aestivum L.)T型细胞质雄性不育系及其保持系的线粒体多肽进行了比较研究,结论如下:1.黄化苗期不育系和保持系线粒体多肽在单向SDS-PAGE和双向IEF-SDS电泳行为上无明显差别;2.在孕穗期幼穗线粒体多肽的单向SDS-PAGE图谱上,两个不育系都缺少28Kd多肽带纹,因而不育系和保持系间表现出明显的差异。双向IEF-SDS凝胶电泳证实28Kd带纹实际上是分子量相同而等电点分别为5.58和5.65的两个多肽;3.线粒体基因的表达是具有时空性质的;4.线粒体与T型细胞质雄性不育可能存在着某种特定的关系。 本文还就T型细胞质雄性不育的分子机制进行了探索性讨论。
Mitochondrial polypeptides from two pair of cytoplasmic male sterile lines and their maintainers in wheat have been fractionated by 2-D IEF-SDS as well as SDS-PAGE. The results are as follows:1. No difference has been found on the chromatograms of SDS-PAGE and 2-D IEF-SDS between mitochondrial polypeptides from etiolated seedlings of CMS lines and their maintainers.2. During the develepment of young ears, one 28Kd polypeptide which virtually appears in two spots on 2-D IEF-SDS is absent from the SDS-PAGE chromatograms of CMS mitochondria, while polypeptide-chromatogrami of maintainers are normal.3. Expression of mtDNA is in order of time and space.4. Mitochondria may involve in CMS.Also, discussion is made on the mechanism of CMS and a tentative model is put forward.
关键词
小麦
胞质雄性不育
线粒体多肽
Whear (T. aestivum L.), CMS Type T., Mitochondrial polypeptides, Electrophoresis
