摘要
目的:为探讨美吡达和胰岛素治疗对2型糖尿病人红细胞膜脂肪酸成分 及膜流动性的影响。方法:应用高效液相色谱（HPLC）及荧光偏振技术测定了采 用美吡达或胰岛素（Ins）治疗的伴和不伴并发症的2型糖尿病患者红细胞膜脂肪 酸成分和膜微粘度,并与正常对照组作了比较。结果:美吡达治疗的病人红细 胞膜花生四烯酸（AA）含量和组成明显低于正常对照（135.84±21.00和144.49±24.33vs 164.54±27.42,P<0.01和P<0.05）,红细胞膜总脂肪酸含量也明显低于正常组和胰岛素（Ins）治疗组（502.94±84.67和505.04±80.40vs 554.69±96.50,均P<0.01）。糖尿病各组红细胞膜微粘度明显高于对照组。而用美毗达治疗伴有并发症组红细胞膜微粘度又明显高于胰岛素组和无并发症组（单纯组）。结论:2型糖尿病患者采用胰岛素治疗有利于维持红细胞膜脂的脂肪酸成分和改善膜流动 性,这在防止和延缓糖尿病微血管并发症的发生和发展方面具有重要的意义。
OBJECTIVE: To study the effect of glipizide and insulin on fatty acid components and fluidity of erythrocyte membrane in type II diabetics. METHODS: The fatty acid components and fluidity of erythrocyte membrane were determined in type 2 diabetic patients with and without complication by HPLC and fluorescence polarization technique. These patients were respectively treated by glipizide and insulin. RESULTS: The contents and composition of erythrocyte membrane arachidonate (C20:4) were significantly lower in two glipizide groups than in controls (135.84±21.00 and 144.49±24.33vs 164.54±27.42,P<0.01 and P<0.05),and the contents of total fatty acids were also lower(502.94±84.67and 505.04±80.40 vs 554.69±96.50,P <0.01). These groups with diabetes had significant increased erythrocyte membrane microviscosities were higher in glipizide group with complicutions than those without complications and insulin-treated patients. CONCLUSIONS :These results suggest that insulin therapy in 2 type diabetes be of help in asserting fatty acid components and improving fluidity in erythrocyte membrane and insulin therapy may play an important role in the initiation and development of diabetic microangiopathy.
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2002年第3期192-194,共3页
The Chinese Journal of Clinical Pharmacology
基金
本文为重庆市应用基础研究基金资助项目.(编号:97-45)
