摘要
目的 :了解喉鳞癌端粒酶活性的表达情况及其与细胞周期蛋白D1(cyclinD1)过度表达的关系。方法 :分别应用TRAP法和免疫组化法对 38例喉鳞癌组织及相关喉粘膜组织的端粒酶活性和cyclinD1的表达情况进行检测。结果 :喉鳞癌病例中 82 % (31/ 38)有端粒酶活性表达 ,5 0 % (19/ 38)有cyclinD1过度表达 ;两者表达均与患者的年龄、肿瘤部位、T分期、病理学分级无关 (P >0 .0 5 ) ,而端粒酶表达与肿瘤的临床分期有关 (P <0 .0 5 ) ,cyclinD1表达与肿瘤的临床分期无关 (P >0 .0 5 ) ,喉正常粘膜中两者均无表达 ;喉鳞癌组织中两者均表达 18例 ,两者密切相关 (P <0 .0 5 )。结论 :端粒酶的激活在喉鳞癌组织中普遍存在 ,cyclinD1过度表达可能是喉鳞癌端粒酶激活的重要机制之一。
Objective:In order to investigate the telomerase activity and its relationship with the overexpression of cyclin D 1 protein in laryngeal squamous cell carcinomas ( LSCC).Method:TRAP(Telomeric repeat amplification protocol) assay and immunohistochemical method were used respectively to detect the telomerase activity and the overexpression of cyclin D 1 protein in 38 LSCC and correspondent laryngeal mucosa.Result:The expressions of telomerase and cyclin D 1 protein were 82%(31/38), 50%(19/38) respectively in LSCC, both of them were not associated with the age of patients, location of tumors, T stage and pathological grade(P> 0.05 ), but there was a significant difference among clnic stages (P< 0.05 ) in telomerase activity, no significant difference could be found among clnic stages in expression of cyclin D 1. 18 out of 31 cases were expressed in both of them simultaneously, there was significant association between them(P< 0.05 ).Conclusion:Telomerase is widely activated in LSCC, the overexpression of cyclin D 1 protein may be one of the most important mechanisms of telomerase activation in LSCC.
出处
《临床耳鼻咽喉科杂志》
CSCD
北大核心
2002年第6期286-288,共3页
Journal of Clinical Otorhinolaryngology
