摘要
Objective: To investigate the effects of nerve growth factor(NGF)on proliferation and DNAthesis of cultured human fetal retinal pigment epithelium (RPE)cells in vitro.Methods: Primary culture and subculture of human fetal retinal pigment epithelium cellswere established in vitro first. Cultured RPE cells were treated with NGF by variousconcentrations 0μg/L, 50μg/L, 100μg/L, 200μg/L and 300μg/L(final concentration)for 48 hs.After 48 hs, cells proliferation was measured with methyl thiazolyl tetrazolium(MTT)assay method and the amount of DNA was determined by the absorbance at 280nm of nucleic acid & protein analysis.Results: The A values of 100 μg/L, 200 μg/L, 300 μg/L NGF was(0. 213 7 ± 0. 23 3),(0. 218 8 ±0. 018 1), (0. 232 2 ±0. 016 4) as compared with(0. 189 7 ±0. 015 2) of Avalue of 0 μg/L NGF respectively, q value was 3.63,4.40, 6. 42 and P value was0. 015, 0. 000, 0. 000(q-test). The DNA concentrations of 100 μg/L, 200 μg/L, 300μg/L and 400 μg/L NGF was (981. 220 4 ± 123.535 7), (1 375. 848 4 ±244. 471 8),(1 658.707 1 ± 176. 938 1), (2 353.086 3 ±609. 906 4) μg/ml as compared with(666. 818 8 ± 141. 330 2) μg/ml of DNA concentration of 0 μg/L NGF respectively, qvalue was 3.63,8.20,11.47,19.46, P value was 0. 024,0. 000,0. 000,0. 000 (q-test).Conclusion: The data suggested that NGF could stimulate the proliferation and DNAsynthesis of cultured of hRPE cells in vitro in a dose-dependent manner.
Objective: To investigate the effects of nerve growth factor(NGF)on proliferation and DNA synthesis of cultured human fetal retinal pigment epithelium (RPE) cells in vitro. Methods : Primary culture and subculture of human fetal retinal pigment epithelium cells were established in vitro first. Cultured RPE cells were treated with NGF by various concentrations 0μg/L, 50μg/L, 100μg/L,200μg/L and 300μg/L(final concentration) for 48 hs. After 48 hs, cells proliferation was measured with methyl thiazolyl tetrazolium (MTT) assay method and the amount of DNA was determined by the absorbance at 280 nm of nucleic acid & protein analysis.Results: The A values of 100 μg/L, 200 μg/L, 300 μg/L NGF was(0. 213 7 ± 0. 23 3), (0.218 8±0. 018 1), (0.232 2 ±0.016 4) as compared with(0. 189 7 ±0.015 2) of A value of 0 μg/L NGF respectively, q value was 3.63,4.40,6.42 and P value was 0.015, 0.000, 0. 000(q-test).The DNA concentrations of 100 μg/L, 200 μg/L, 300 μg/L and 400μg/L NGF was (981. 220 4 ±123. 535 7), (1375.8484 ± 244.4718), (1 658. 707 1±176. 938 1), (2 353. 086 3±09. 906 4) μg/ml as compared with (666. 818 8 ±141. 330 2) μg/ml of DNA concentration of 0 μg/L NGF respectively, q value was 3. 63,8. 20,11. 47,19. 46, P value was 0. 024,0. 000,0. 000,0. 000 ( q-test). Conclusion: The data suggested that NGF could stimulate the proliferation and DNA synthesis of cultured of hRPE cells in vitro in a dose-dependent manner.
关键词
眼色素上皮
神经生长因子
增殖
DNA合成
pigment epithelium of eye, nerve growth factor, proliferation, DNA synthesis
