摘要
目的 通过测量糖基化终产物对培养的牛视网膜毛细血管周细胞抗氧化物酶和脂质过氧化物含量的影响 ,以探讨氧化应激在糖尿病视网膜病变进程中的作用。 方法 不同浓度的糖基化终产物(0 ,8,32 ,12 5 ,5 0 0 ,2 0 0 0μg/ ml)与周细胞作用 4 d后 ,以分光光度法测量细胞内过氧化氢酶的活性及过氧化脂质丙二醛的含量。 结果 糖基化终产物能以剂量依赖的方式降低周细胞内过氧化氢酶的活性 (r=- 0 .714 ,P<0 .0 1) ,增加丙二醛的含量 (r=0 .74 8,P<0 .0 1) ,与对照组相比 ,当糖基化终产物浓度达到32 μg/ ml时 ,两者比较差异有显著性的意义 (P<0 .0 1)。 结论 氧化应激的增加可能是早期糖尿病视网膜病变中周细胞丧失的原因之一。
Objective To investigate the effect of advanced glycation end products (AGEs) on the catalase activity and the levels of malondialdehyde in cultured bovine retinal capillary pericytes (BRPs), and to investigate the relationship between oxidative stress and diabetic retinopathy. Methods Cultured BRPs were exposed to AGEs (0, 8, 32, 125, 500, 2 000 μg/ml) for four days. Activity and the levels of catalase and malondialdehyde in cultured BRPs were examined by spectrophotometry. Results AGEs decreased the catalase activity, whereas increased the levels of malondialdehyde of cultured BRPs in a dose dependent manner ( r=-0.714, r=0.748, P< 0.01).There were significant differences between BRPs cultured in 32 μg/ml AGEs and in control group ( P< 0.01), while no significant differences between BRPs cultured in non glycated bovine serum albumin and absence of bovine serum albumin were found. Conclusion Oxidative stress may be one of the reasons why the pericyte disappears in diabetic retinopathy.
出处
《中华眼底病杂志》
CAS
CSCD
2002年第2期143-145,共3页
Chinese Journal of Ocular Fundus Diseases
