摘要
目的 研究肝硬化大鼠血浆一氧化碳 (CO)水平与血流动力学改变之间的关系。方法 将SD大鼠分为两组 ,四氯化碳肝硬化模型组 (n =10 )及正常对照组 (n =10 ) ,用联二亚硫酸盐还原法测定血浆CO的含量 ,镉柱还原法测定血浆一氧化氮 (NO)的含量 ,根据胆红素的生成量测定组织血红素氧合酶 (HO)的活性 ,插管法测定血压、心率及门静脉压力。结果 与对照组相比 ,肝硬化组血浆CO、NO水平显著升高 [(18.6 9± 1.86 ) μmol/L ,(43.12± 3.2 5 ) μmol/L比 (10 .2 7± 1.2 1) μmol/L ,(2 7.75±2 .72 ) μmol/L ,P均 <0 .0 1],门静脉压力 (PP)亦明显升高 [(16 .6 7± 0 .6 3)cmH2 O比 (8.82± 0 .2 9)cmH2 O ,P <0 .0 1],平均动脉压 (MAP)明显减低 [(15 .92± 0 .74 )kPa比 (18.93± 0 .71)kPa ,P <0 .0 1]。肝硬化组血浆CO、NO水平与MAP呈负相关 (r =- 0 .6 7,- 0 .74 ;P <0 .0 5 ,P <0 .0 1)。肝硬化组脾脏HO活性升高 80 % ,小肠HO活性升高 10 0 % ,肠系膜组织HO活性升高 330 % ,而肝脏、肾脏HO活性变化不明显。结论 CO作为一种新信号分子 ,可能参与肝硬化高动力循环的发生。
Objective To explore the relationship between the plasma level of carbon monoxide and the hyperdynamic circulation of cirrhotic rats. Methods S.D. rats were divided into cirrhotic ( n =10) and control ( n =10) groups. Liver cirrhosis was induced by percutaneous administration of carbon tetrachloride. Mean arterial pressure (MAP, kPa),heart rate (HR,beat/min) and portal pressure (PP,cm H 2O) were measured by indwelling catheters. Plasma CO was measured by Chalmers method, and plasma NO 2/NO 3 was determined by the reduction of cadmium column. Heme oxygenase (HO) activity in splanchnic organs from cirrhotic rats and controls was also determined. Results Typical features of cirrhosis were histologically observed in carbon tetrachloride treated animals. The cirrhotic group presented a significant increase in plasma CO [( 18.69 ± 1.86 )μmol/L vs. ( 10.27 ± 1.21 )μmol/L, P < 0.01 ], plasma NO [( 43.12 ± 3.25 )μmol/L vs. ( 27.75 ± 2.72 )μmol/L, P < 0.01 ], and PP [( 16.67 ± 0.63 ) vs. ( 8.82 ± 0.29 ) cm H 2O, P < 0.01 ] but a significant decrease in MAP [( 15.92 ± 0.74 )kPa vs. ( 18.93 ± 0.71 )kPa, P < 0.01 ] in comparison with the controls. No differences were observed regarding HR. In a single regression analysis, significant correlations were detected between plasma CO and NO ( r = 0.57 , P < 0.05 ), between plasma CO and MAP ( r= -0.67 ,P< 0.05 ), and between plasma NO and MAP ( r= -0.74 ,P< 0.01 ). In comparison with controls, the activity of HO was significantly increased in the spleens (80% increase), intestines (100% increase), and mesenteries (330% increase) from cirrhotic rats. Conclusions Carbon monoxide may act as a new messenger to play a role in hyperdynamic circulation of cirrhotic rats.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2002年第7期401-403,共3页
Chinese Journal of Digestion
