摘要
目的 研究榄香烯对喉癌细胞的作用及机制。 方法 用MTT法测定榄香烯对喉癌细胞的抑制作用 ,在透射电镜下观察其形态学变化 ,用流式细胞仪研究不同浓度、不同时间细胞凋亡的发生和对细胞周期的影响。 结果 榄香烯明显抑制Hep_2细胞的生长 ,对Hep_2细胞的半数生长的抑制剂量 (IC50 )值 2 4h为 6 1.3μg ml。透射电镜观察发现染色质浓缩 ,沿着核膜排列 ,形成不同形状和大小的块状。流式细胞术证实 ,凋亡细胞具有时间和药物浓度双相依赖性。榄香烯阻滞Hep -2细胞从S期进入G2 M期。 结论 在实验室条件下 ,榄香烯能够抑制喉癌细胞的生长 ,其抑制作用与细胞凋亡有关。
Objective To study the effects of elemene on laryngeal neoplasmic cells and its mechanism. Methods The effect of elemene on the growth of laryngeal neoplasmic cells was evalua_ ted by MTT assay. The morphological alterations were confirmed by electron microscopy. Using flow cytometry, we investigated the Hep-2 cells in different conditions. Results The growth of Hep-2 cells was significantly inhibited by elemene, The IC 50 value was 61.3μg/ml. The marked morphological changes included condensation of chromatin arranged under the nuclear membrane. Apoptotic cells increased with the increase of drug concentrations and the elapse of time. The cell cycle was arrested from S to G 2M phase transition . Conclusion The growth of Hep-2 cells can be inhibited by elemene. Under experimental conditions, induction of apoptosis contributes to the anticancer activity of elemene.
出处
《上海第二医科大学学报》
CSCD
2002年第4期306-308,共3页
Acta Universitatis Medicinalis Secondae Shanghai
