摘要
0 .0 1mol/LHepes,pH 7.4,室温条件下 ,以酒石酸为脱除剂 ,监测铽 (Ⅲ )与脱铁转铁蛋白结合的两种配合物C端单铽转铁蛋白和N端单铽转铁蛋白随酒石酸浓度变化的脱除动力学 ,根据其动力学行为 ,我们推测存在两种平行的脱除途径 :一次途径和饱和途径 ,其中C端单铽转铁蛋白的铽 (Ⅲ )脱除呈现饱和与一次相结合途径 ,N端单铽转铁蛋白为简单的一次途径 .NaCl的加入可促进两种单铽转铁蛋白铽 (Ⅲ )的脱除 。
The rates at which terbium(Ⅲ) ion is removed from the two metal binding sites of human serum transferrin by tartaric acid have been evaluated. Solutions are buffered at pH 7.4 by 0.01 mol/L N-2-hydroxy-ethylpiperazine-N′-2-ethanesulfonic acid (Hepes) and maintained at room temperature. According to their kinetic behaviors, it is proposed that there are two parallel removal pathways. One is saturation pathway, the other is first-order pathway. The results show that terbium(Ⅲ) ion removal is through both pathways for the C-terminal site, while through simple first-order pathway for the N-terminal site. The addition of sodium chloride to the buffer accelerates terbium(Ⅲ) ion removal from both forms of monoterbium transferrin, and the C-terminal monoterbium transferrin is more easily affected by sodium chloride.
出处
《化学学报》
SCIE
CAS
CSCD
北大核心
2002年第7期1253-1257,共5页
Acta Chimica Sinica
基金
国家自然科学基金 (No .2 0 0 710 2 2 )资助项目
