摘要
目的观察黄芪对人成纤维细胞ICAM_1、CD126 表达及合成细胞外基质(ECM)的影响 ,探讨其抗肝纤维化的机制。方法采用细胞培养法 ,于细胞培养液中加入不同浓度的药物 ,通过流式细胞仪观察经分子探针标记的ICAM_1、CD126 阳性细胞数变化 ,用放免法测定培养上清HA、LA含量。结果人成纤维细胞表达ICAM_1、CD126 阳性率分别为(82 63±5.94) %和(77.95±4.68) %,加入黄芪组二者阳性率明显下降。但黄芪对成纤维细胞合成HA无影响 ,对LN影响较小。结论黄芪能抑制成纤维细胞表达ICAM_1和CD126,这可能是其抗肝纤维化机理之一 ,它对合成ECM影响较小。
Aim To explore the anti-fibrotic mechanism of Radis Astragali, we observed the ICAM-1 and CD126 expression affected by Radis Astragali in human fibroblasts.Methods Different dosages of Radis Astragali were added in the mediums for fibroblast culture.The ICAM-1 and CD126 positive cells were assessed by using flow cytometry.Results Positive rate of ICAM-1 and CD126 in normal cultural fibroblasts was 82.63±5.94 and 77.95±4.68 respectively. It decreased in cultural fibroblasts with Radis Astragali.Conclusion Radis Astragali can inhibit the immune-adhensive function of fibroblasts, which may-be one of its anti-fibrotic mechanisms.
出处
《中国临床药理学与治疗学》
CAS
CSCD
1999年第3期209-210,共2页
Chinese Journal of Clinical Pharmacology and Therapeutics
