摘要
目的 探讨约氏疟原虫感染早期Th1反应对再感染的影响。方法 将约氏疟原虫感染早期的部分小鼠用蒿甲醚治愈后 ,对治疗和未治疗而自然自愈的小鼠经腹腔再次感染约氏或伯氏疟原虫 ;应用吉姆萨薄血膜染色法观察并计数两者的红细胞感染率 ;并通过ELISA法检测其脾细胞培养上清中的细胞因子和血清中的抗体水平。结果 自然自愈的小鼠用约氏疟原虫再感染 ,没有虫体血症产生 ,而用伯氏疟原虫感染则产生了低水平的虫体血症 ;蒿甲醚治愈的小鼠对约氏和伯氏疟原虫的再感染均产生了较低的虫体血症 ;初次感染早期脾细胞培养上清中的IFN -γ水平显著升高 ,与此相比 ,约氏疟原虫再感染的小鼠脾细胞可产生更高水平的IFN -γ。结论 疟原虫感染早期Th1反应的建立在抗疟原虫再感染过程中发挥重要的作用 ;
Aim To investigate the effects of Th1 response in the early phase of plasmodium infection on reinfection Methods Some mice,infected intraperitoneally by P yoelii,were treated with Artemether Tablet in early phase of infection All of mice,including treated and untreated mice,were reinfected with P yoelii or P berghei;both parasitemias were observed by microscopy on Giemsa′s-stained thin blood smears;the levels of cytokines in culture supernatants of splenocytes and antibodies in sera were measured with ELISA Results In nature recovery mice,parasitemia was not found after reinfection with P yoelii,but lower parasitemia was observed after infection with P berghei;comparing with control group,there was lower parasitemia in the mice treated with Artemether Tablet after reinfection with P yoelii or P berghei IFN-γ levels in culture supernatants of splenocytes went up gradually in the early phase of primary infection,but they became higher than that of primary infection after reinfection with P yoelii Conclusion The effects of Th1 response in the early phase of plasmodium infection are very important to resist plasmodium reinfection;the morbidities of reinfection by homologous and heterologous plasmodium or the development of disease are closely related to the parasitemia level of primary infection
出处
《中国人兽共患病杂志》
CSCD
北大核心
2002年第4期68-70,共3页
Chinese Journal of Zoonoses
