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GC–MS analysis of bioactive compounds present in different extracts of an endemic plant Broussonetia luzonica (Blanco) (Moraceae) leaves

GC–MS analysis of bioactive compounds present in different extracts of an endemic plant Broussonetia luzonica (Blanco) (Moraceae) leaves
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摘要 Objective: To investigate and characterize the chemical composition of the different crude extracts from the leaves of Broussonetia luzonica(Blanco)(Moraceae)(B. luzonica), an endemic plant in the Philippines.Methods: The air dried leaves were powdered and subjected to selective sequential extraction using solvents of increasing polarity through percolation, namely, n-hexane,ethyl acetate and methanol to obtain three different extracts. Then, each of the extracts was further subjected to gas chromatography–mass spectrometry.Results: Qualitative determination of the different biologically active compounds from crude extracts of B. luzonica using gas chromatography–mass spectrometry revealed different types of high and low molecular weight chemical entities with varying quantities present in each of the extracts. These chemical compounds are considered biologically and pharmacologically important. Furthermore, the three different extracts possess unique physicochemical characteristics which may be attributed to the compounds naturally present in significant quantities in the leaves of B. luzonica.Conclusions: The three extracts possess major bioactive compounds that were identified and characterized spectroscopically. Thus, identification of different biologically active compounds in the extracts of B. luzonica leaves warrants further biological and pharmacological studies. Objective: To investigate and characterize the chemical composition of the different crude extracts from the leaves of Broussonetia luzonica(Blanco)(Moraceae)(B. luzonica), an endemic plant in the Philippines.Methods: The air dried leaves were powdered and subjected to selective sequential extraction using solvents of increasing polarity through percolation, namely, n-hexane,ethyl acetate and methanol to obtain three different extracts. Then, each of the extracts was further subjected to gas chromatography–mass spectrometry.Results: Qualitative determination of the different biologically active compounds from crude extracts of B. luzonica using gas chromatography–mass spectrometry revealed different types of high and low molecular weight chemical entities with varying quantities present in each of the extracts. These chemical compounds are considered biologically and pharmacologically important. Furthermore, the three different extracts possess unique physicochemical characteristics which may be attributed to the compounds naturally present in significant quantities in the leaves of B. luzonica.Conclusions: The three extracts possess major bioactive compounds that were identified and characterized spectroscopically. Thus, identification of different biologically active compounds in the extracts of B. luzonica leaves warrants further biological and pharmacological studies.
出处 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2016年第11期957-961,共5页 亚太热带生物医学杂志(英文版)
基金 supported by Faculty of Pharmacy and Office of Grants, Endowments and Partnerships in Higher Education University of Santo Tomas, Manila, Philippines Nanocast Lab, Department of Chemistry, Mahidol University, Bangkok, Thailand Department of Science and Technology, Philippines Commission on Higher Education, Philippines
关键词 Broussonetia luzonica(Blanco) Selective sequential extraction GC–MS analysis ENDEMIC PERCOLATION Broussonetia luzonica(Blanco) Selective sequential extraction GC–MS analysis Endemic Percolation
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