摘要
以人红白血病K5 6 2细胞株为材料 ,应用基因芯片技术研究苦丁茶甙对人红白血病K5 6 2细胞作用前后基因表达的差异 ,以诱导分化药物羟基脲处理作为正对照 ,分别提取药物处理前后细胞RNA进行逆转录cDNA ,使获得的cDNA分别标记上Cy3和Cy5两种荧光物质 ;然后与由 16 32条cDNA片段制作的基因表达谱芯片杂交 ,经扫描及对获得的数据用相关软件分析 ,确定K5 6 2细胞经苦丁茶甙处理前后差异表达基因 4 8条 ,有 4 1条与羟基脲处理相同 ,其中上调表达的基因有 32条 ,下调表达的基因有 16条 .
The application of cDNA microarray technology for studying the gene expression in Leukemia K562 cells,which were treated with kudingcha glycoside.mRNAs were isolated from the cells treated with kudingcha glycoside or hydroxyurea,which could introduce K562 cell differentiation and was used as positive contrast,and then reverse transcribed to cDNas.The cDNAs labeled with two kinds of fluorescence materials Cy3 dUTP of Cy5 dUTP,respectively and cohybridized with the microarray containing 1 632 cDNA fragments.The microarray was scanned and the data was analyzed by software.The 48 differentially expressed genes were identified after kudingcha glycoside treatment.Among them,41 genes were the same as treatment with hydroxyurea.In those genes,32 were up regulated expressed and 16 were down regulated expressed.
出处
《华南理工大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2002年第7期83-86,共4页
Journal of South China University of Technology(Natural Science Edition)
基金
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