摘要
研究了诱导棉花花粉萌发的不同培养基,比较和分析了不同培养基和不同培养方法的优劣,分别应用添加本品种柱头和花瓣提取液以及综合氨基酸液的改良Taylor培养基,首次获得了能比较长时间保持其生活力和长势旺盛的萌发花粉。对花朵进行低温处理,取得了提高花粉萌发率的良好效果。讨论了低温促进萌发和蓖麻油造成无效朗发的原因。将诱导萌发的培养基涂于母本离体柱头上,得到促进种间离体受精的良好效果。
The effects of different media and cultural methods on cotton pollen germination were compared. Pollen germination was successful on the improved Taylor solid medium in which the extracts of the stigmata and petals of the original species, and the comprehensive amino acids were added, respectively. The germinating pollens were viable, and active protoplasmic streaming was observed within the pollen tube. It was found that low temperature pretreatment could increase the pollen germination rate. The reasons that low temperature pretreatment stimulated pollen germination and castor oil caused abortive germination of cotton pollens were discussed. In vitro fertilization between two species of Gossypium can be promoted by sm- earing the stigmata with the improved Taylor medium.
关键词
棉花
花粉
萌发
离体受精
Cotton
Artificial pollen germination
In vitro fertilization
