摘要
目的 :观察含硒藻蓝蛋白 (Se -SPC)对四氯化碳 (CCl4)致小鼠急性肝损伤的拮抗作用。方法 :以 2 %CCl4油灌胃复制小鼠急性肝损伤模型 ,各组分别腹腔注射Se -SPC、藻蓝蛋白 (SPC)、无机硒 (Se) 7d ,测定血及肝组织中Se、谷胱甘肽过氧化物酶 (GPx)、超氧化物歧化酶 (SOD)、丙二醛 (MDA)、谷丙转氨酶 (ALT)、一氧化氮 (NO)水平 ,分析Se -SPC、SPC、无机Se对上述指标的影响。结果 :Se -SPC组比CCl4组血和肝中Se、GPx、SOD水平显著增高 (P<0 0 5 ) ,血ALT、血和肝中MDA及NO-2 /NO-3 水平显著降低 (P <0 0 5 ) ,高剂量Se -SPC组对Se含量、GPx、MDA、NO-2 /NO-3 等指标的影响更为显著 (P <0 0 1) ,在相同蛋白或硒剂量下 ,Se-SPC组比SPC组和无机Se组对上述指标作用更大。相关分析发现小鼠血Se水平与GPx活性呈显著正相关 (r=0 70 5 ) ,血GPx活性与MDA、NO-2 /NO-3 、ALT水平呈明显负相关 (r=- 0 6 2 9,r =- 0 336 ,r=- 0 4 5 7) ,血ALT活性与MDA及NO-2 /NO-3 呈显著正相关 (r=0 5 19,r=0 6 4 1)。结论 :Se -SP可能结合增高硒酶活性及抗炎双重功效 。
AIM: To investigate the effect of Se-containing spirulina phycocyanin (Se-SPC) on liver injury of mice induced by carbon tetrachloride (CCl 4). METHODS: The mouse model was conducted by intragastric feeding with 2% CCl 4 oil for three times, meanwhile Se-SPC, spirulina phycocyanin (SPC) and Na 2SeO 3 were injected (ip) to various groups for 7 days. Then selenium (Se), glutathione peroxidase (GPx), superoxide dismutase (SOD), alanine aminotransferase (ALT), malondiaoldehyde (MDA) and nitric oxide (NO) levels in blood and liver were measured. RESULTS: The level of Se, GPx and SOD activities were obviously higher ( P< 0.05) but ALT activity, MDA and NO - 2/NO - 3 levels were remarkably lower ( P< 0.05) in Se-SPC treated groups than those in CCl 4 groups, and effects of high dose Se-SPC on Se, GPx, MDA and NO - 2/NO - 3 were even more significant ( P <0 01). Under the same dose of Se or protein, effects of all selected targets in Se-SPC groups were more efficient than those in SPC groups and inorganic-Se groups. Furthermore, Se levels had a positive correlation with GPx activity ( r= 0 705), which had negative correlation with levels of MDA, NO - 2/NO - 3 and ALT ( r =-0 629, r =-0 336, r =-0 457, respectively ), and positive correlations between ALT activity and MDA or NO - 2/NO - 3 level were found ( r= 0 519, r= 0 641 ). CONCLUSION: These results indicated that Se-SPC may attenuate liver injury of mice induced by CCl 4 through its anti-inflammatory action and enhancing selenoenzyme expression.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2002年第7期819-822,共4页
Chinese Journal of Pathophysiology
基金
广东省重点科技攻关项目 (1996 - 0 4 - 0 6 )
广州市科技项目 (2 0 0 1-J - 0 0 1- 0 1)
