摘要
利用四环素抗性基因启动子在金色链霉菌中表达透明颤菌血红蛋白基因。在 1m3 发酵罐中研究了工程菌株的生长代谢特性。在溶解氧充足的条件下 ,透明颤菌血红蛋白表达 ,对金色链霉菌生长代谢未产生明显影响 ,工程菌株与参比菌株的生长代谢特性基本一致 ,工程菌株和参比菌株金霉素最终浓度分别为 2 2 90 5u mL、2 2 896u mL。在低溶解氧条件下 ,透明颤菌血红蛋白的表达 ,可促进金色链霉菌菌体生长、菌丝活力保持和金霉素的合成 :工程菌菌体浓度比参比菌株高 5 %～ 1 0 % ,产物合成提高 1 1 4%。
Vitreoscilla hemoglobin Gene was expressed in S.aureofaciens through promoter of tetracycline resistance gene.Characteristics of S.aureofaciens growth and metabolism were studied in 1m\+3 fermenter.In high dissolved oxygen concentrations,expression of hemoglobin gene had little effect on growth and metabolism of S.aureofaciens ,and there were no obvious differences between engineering strain and control.The chlortetracycline of engineering strain was 22905 u/mL and of control was 22896u/mL respectively.Under conditions of low dissolved oxygen,expression of hemoglobin enhanced growth,maintenance of energetic mycelium configuration and chlortetracycline yield of S.aureofaciens :the mycelium concentrations of engineering strain were more about 5%～10% and yield was more 11.4% than control.
出处
《微生物学报》
CAS
CSCD
北大核心
2002年第4期418-424,共7页
Acta Microbiologica Sinica
基金
福建省科委资助项目 ( 99-H -4 6 )~~
关键词
透明颤菌
血红蛋白
基因表达
金色链霉菌
生长代谢
溶解氧浓度
金霉素
Vitreoscilla hemoglobin Gene, Streptomyces aureofaciens , Dissolved oxygen concentration, Chlortetracycline
