摘要
通过PCR方法克隆了从哈尔滨分离的一株鸡贫血病毒 (CAV)的全基因 ,并对其进行了测序 ,该病毒基因组为环状 ,全长 2 2 98bp ,含有三个互相重叠的开放读码框和一个调控区。将克隆的基因与GenBank收录的CAV基因比较 ,同源性至少为 97% ,未发现与本次克隆的CAV基因完全一致的分离株。与德国分离株Cux la、2 6p4和马来西亚分离株分别有 42、42和72个核苷酸不同 ,同源性分别为 98 2 %、98 2 %和 96 9%。与德国分离株Cux 1b相比 ,除在调控区内少一个类似增强子的重复序列外 ,尚有 3 9处核苷酸不同。它与分离于欧洲的几株CAV的亲源性要比来自亚洲的马来西亚株近。对CAV哈尔滨分离株、2 6p4、Cux 1b、Cux 1a和马来西亚株的VP1、VP2和VP3蛋白比较 。
A Chicken anemia virus has been isolated from a chicken flock in Harbin of China.The genome of the ivrus was cloned through polymerase chain reaction(PCR) and sequence of the genome was analyzed.The cycle genome is made of 2298 base pairs including three overlapping open reading frames(vp1,vp2,vp3)and a regulative region.Comparing sequence of the genome through BLAST in GenBank,this sequence exhibits 96.9% identity with other genome of CA Vs and least.Multiple alignment of this genome of this virus, 26p4,strain isolated in Germany,strain isolated in Malaysia and Cux 1 found that this sequence exhibits 98.2%(42/2298),98.2%(42/2298),96.9%(72/2298)and 97.5%(60/2319)identify with them,respectively.A new CAV strain was isolated and it has better identify with CAV isolated in Europe countries than is Asia country Malaysia.Multiple alignment of VP1,VP2,VP3 of 26p4,strain isolated in Germany,strain isolated in Malaysia,Cux 1 and strain isolated in Harbin of China found the VP2 the most conservative.
出处
《微生物学报》
CAS
CSCD
北大核心
2002年第4期436-441,共6页
Acta Microbiologica Sinica
基金
黑龙江科委攻关项目 :编号 97SG74SG0 4
