摘要
目的:探讨白细胞在早期糖尿病大鼠视网膜微血管中粘附、堆积,以致引起微循环障碍的作用。方法:健康成年雄性Wistar大鼠16只,随机分成正常对照组和链尿佐菌素（Streptozotocin,STZ）糖尿病3个月组各8只;对其视网膜消化铺片进行形态学观察及白细胞共有抗原（CD45）单克隆抗体免疫组织化学研究。结果:发现3个月病程的STZ诱导的糖尿病大鼠视网膜毛细血管已出现毛细血管管径不规则,呈节段性膨大或囊样扩张等形态学改变;视网膜毛细血管中CD45的表达明显增加,白细胞呈串珠样粘附和堆积。结论:3个月病程的STZ糖尿病大鼠可出现视网膜微血管改变;白细胞在糖尿病视网膜病变的发生发展中起着重要作用。
Purpose: To explore the leukocytic functions in adhesion, stacking and causingmicrocirculatory disorder in retinal microvessels in early diabetic rats.Methods: Sixteen healthy adult male Wistar rats were randomly divided into normalcontrol group and 3 months group of diabetes (STZ) . We carried out morphologicalobservations and CD45(Leukocyte Common Antigen) monoclonal antibody immunohist-ochemical studies of the retinal digest preparations.Results: Morphological changes including capillary varix, irregularity of capillarycaliber, was found in the retinal blood capillaries in diabetic rats at 3 months. Furthermore, the expression of CD45 was significantly increased in the diabetic retinalmicrovessels, which were found leukocytes adhering and stacking.Conclusion: Changes of retinal microvessels can be found in STZ diabetic rats at 3months. The leukocytes were important in the onset and development of diabeticretinopathy(DR).
出处
《眼科学报》
2002年第2期71-75,共5页
Eye Science
基金
本研究受"广东省眼科视觉科学重点实验室"专项基金资助(B60105)
