摘要
应用PALLAS将病毒粒体转化为乳胶颗粒通过扫描电镜直接观察,成功地检测到了大豆花粉表面污染的大豆花叶病毒(SMV)。田间春大豆宁镇1号的重花叶、轻花叶及无症状植株花粉所吸附的乳胶颗粒依次递减,而健株花粉上极少观测到乳胶颗粒,花粉数量测定及萌发试验表明,SMV的侵染对大豆花粉形成及活力都有明显的影响。
The particles of soybean mosaic virus on the surface of soybean pollen were detected by protein A-linked latex antiserum(PALLAS)with scanning microscope When soybean variety of Ning Zhen -1 was used as material, the latex beads on pollen from severe mosaic, light mosaic and symptomless soybean plants were about 1000, 200 and 15 respectively, but no latex beads were observed on the surface of pollen from the healthy one. Pollen germination experiment shows that the pollen activity is obviously reduced because of the SMV infection.
出处
《中国病毒学》
CSCD
1991年第3期232-235,共4页
Virologica Sinica
关键词
大豆花叶病毒
花粉
PALLAS
污染
Soybean mosaic:virus Pollen PALLAS Scanning electron microscopy Pollcn germination
