摘要
本研究观察乌梢蛇水解液对大鼠胶原性关节炎(CIA)的作用。实验分预防性和治疗性疗效观察两部分,均设低剂量组(0.5 mg/kg)、中剂量组(5 mg/kg)、高剂量组(15 mg/kg)和空白对照组,预防性疗效观察于大鼠致炎前14、11、8、5和2d,给药共5次;治疗性疗效观察于大鼠致炎后第18天,将患关节炎的大鼠分组,每天给药一次,共21d。分别于预防给药大鼠致炎后第25天和治疗给药大鼠致炎后第39天记录各组大鼠关节炎的发病率、关节炎症状指数、血清Ⅱ型胶原抗体水平和皮肤对Ⅱ型胶原的迟发型超敏反应(DTH)。结果表明预防给药中剂量和高剂量乌梢蛇水解液能降低大鼠CIA的发病率、改善关节炎症状(P<0.05),治疗给药中剂量和高剂量能减轻关节炎症状(P<0.05);低剂量无预防发病率和治疗作用。中、高剂量药物均能降低预防和治疗给药CIA的血清抗Ⅱ型胶原抗体水平和皮肤对Ⅱ型胶原的DTH。实验提示乌梢蛇水解液对大鼠CIA有预防和治疗作用。
The aim of this study is to find out the therapeutic and prophylactic effect of a Chinese medicine Zaocys dhumnades (Cantor )hydrolysates on collagen-induced arthritis. Experiments were set up with prophylactic and therapeutic groups in which the low-dose (0.5 mg/kg ), middle-dose (5 mg/kg ) and high-dose (15 mg/kg ) groups as well as the control groups were included. The drugs were given into rats on 14, 11, 8, 5 and 2 days before the induction of ex-perimental arthritis in the prophylactic group. in the therapeutic group, the drugs were given daily for 21 days since 18th day after induction of arthritis. The incidence rates and index of arthritis were recorded and the level of antibodies to type II collagen ( CII ) and the diameters of cutaneous DTH reactions were detected at 25th day after induction of inflammation in the therapeutic group, and at 39* day in the prophylactic group. Experimental results showed that this Chinese medicine in prophylactic middle and high dosages could reduce the incidence of arthritis and improve the symptoms, while its therapeutic middle and high dosages could lessen the symptoms. in the prophylactic and therapeutic groups, the levels of antibodies to CII and the diameters of cutaneous DTH were also reduced. In case of using the middle and high dosages of drugs. The low-dose usage of drug showed neither prophylactic nor therapeutic effects. The results of this study showed that Zaocys dhumnades (Cantor ) hydrolysate has prophylactic and therapeutic effects on CIA.
出处
《上海免疫学杂志》
CSCD
北大核心
2002年第4期257-259,229,共4页
Shanghai Journal of Immunology
基金
上海市医学特色专科建设重点资助项目(No.92-11-008)
