摘要
目的分析2010～2012年深圳市龙岗区致感染性腹泻沙门菌的流行特征及脉冲场凝胶电泳（PFGE）分子分型，为沙门菌病的防控提供参号依据。方法对147株沙门菌进行生化鉴定和血清分型，利用PFGE进行分子分型，采集相关的流行病学信息并分析。结果龙岗区2010～2012年沙门菌的流行具有明显季节性，第i季度为高峰期（46．3％，96／147）；患者主要为婴幼儿（27．9％，41／147）和青壮年（23．1％，37／147），男女比例为1．37：1（85／62）。147株沙门菌分属于33种血清型．肠炎沙门菌（35．4％，52／147）和鼠伤寒沙门菌（28．6％，42／147）为优势血清型，XbaⅠ酶切将其分为105种带型，肠炎沙门菌和鼠伤寒沙门菌分别有17和34种PFGE型别，优势分子型别分别是XE5、XE1和XP8。AvrⅡ酶切显示本地区可能有数起小型暴发。结论深圳市龙岗区2010～2012年沙门菌PFGE型别较多，菌株来源复杂，主要旱散发状态，偶有局部小型暴发，夏秋季要做好学龄前儿童和青壮年沙门菌病的防控工作。
Objective Pulsed-field gel electrophoresis （PFGE） was used to study the epidemiological characteristics and molecular phenotypes of Sab, Jonella in the Longgang District of Shenzhen from 2010 to 2012 in order to provide a scientific basis for prevention and treatment of Salmonella infections. Methods A total of 147 isolates from a system for clinical surveillance of infectious diarrhea were identified using biochemical tests and serotyping and were further typed using PFGE. Agar diffusion as recommended by the WHO （the KB method） was used to test for antibiotic resistance. BioNumerics software was used to analyze the PFGE results in order to determine the correlation between different Salmonella strains. Relevant epidemiological information was also collected and analyzed. Results The prevalence of Salmonella from 2010 to 2012 had obvious seasonality. Incidence peaked in autumn （46. 3%, 96/147）. Salmonella infection was found in all age groups, and mostly in infants （27. 9%, 41/147） and young adults （23. 1%, 37/＇147）. The sex ratio of patients （male： female） was 1.37%1 （85/62）. Three strains of the Salmonella isolates could not be serotyped, but the oth er 144 Salmonella isolates belonged to 33 serotypes. Salmonella enteritidis （35. 4% , 52/147） and Salmonella typhi murium （28.60/00, 42/147） were the predominant serotypes. In total, 105 PFGE profiles were identified using Xba I digestion and represented great genetic diversity. S. enteritidis isolates had 3 predominant PFGE patterns （XES, XE1, and XPS） while S. typhimurium isolates had 1 predominant pattern （XPS）. Conclusion The numerous PFGE patterns of Salmonella from 2010 to 2012 reflected the diversity of these isolates. Cases were mainly sporadic and involved small occasional outbreaks. Prevention and control efforts should focus on infants and young adults in the summer and autumn.
出处
《中国病原生物学杂志》
CSCD
北大核心
2014年第6期519-522,共4页
Journal of Pathogen Biology
基金
2012年深圳市科技计划项目(No.201203347)
关键词
沙门菌属
脉冲场凝胶电泳
分子分型
Salmonella enleritidis
pulsed-field gel electrophoresis （PFGE）
molecular typing
