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微藻培养条件优化研究

Optimization Study on Microalgae Culture Conditions
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摘要 微藻生物柴油是生物能源领域中很有前景的一个重要方向。本文利用微藻(Chlorella Protothecoides)为研究对象,以BG培养基为底物进行液态培养,培养后用离心干燥后得干微藻。用浊度法和干重法对微藻生长情况进行测定,对微藻培养的最优培养基、接种量和培养温度等进行研究。研究表明微藻培养最适接种量1∶5、初始pH值5.7、培养温度30℃和BG+2g/LC6H12O6培养基。 Microalgae biodiesel is an important promising direction in bio- energy field. In this paper,microalgae is used as the research object,and BG medium is used as the substrate to be cultured in liquid,then the dried microalgae is obtained by centrifugation. The microalgae growth is measured with turbidity and dry weight method. The optimal medium for microalgae culture,inoculation and incubation temperature is studied. It is shown in the study that the optimum inoculum is 1∶ 5,initial pH is 5. 7,temperature is 30 ℃ and BG + 2g /LC6H12O6 medium.
出处 《应用能源技术》 2014年第7期15-19,共5页 Applied Energy Technology
关键词 生物柴油 微藻 液体培养 Biodiesel Microalgae Liquid culture
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